ArticleMolecules (Basel, Switzerland)2023
Chromogenic Assay Is More Efficient in Identifying α-Amylase Inhibitory Properties of Anthocyanin-Rich Samples When Compared to the 3,5-Dinitrosalicylic Acid (DNS) Assay.
Article in Molecules (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 15 citations in OpenAlex.
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- Anti-Hyperglycemic and Antioxidant Effects ofInternational journal of molecular sciences · 2026Article
- α-Glucosidase Inhibitory Potential ofFood science & nutrition · 2026Article
- Functional characterization of Lytic Polysaccharide Monooxygenase from Enterococcus mundtii CRi_37 for enhanced stubble saccharification.Molecular biology reports · 2025Article
- Enhanced Bioactive Peptide Release from Pre-Hydrolysed Pea Protein: Impact of Pepsin Digestion on Antidiabetic and Antihypertensive Functions.Foods (Basel, Switzerland) · 2025Article
- In-vitro investigation of antidiabetic and antioxidants properties of major prebiotics and plant based dietary fibers.Journal of diabetes and metabolic disorders · 2025Article
- Unraveling the Impact ofFoods (Basel, Switzerland) · 2024Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The inhibition of carbohydrate digestion by plant bioactive compounds is a potential dietary strategy to counteract type 2 diabetes. Indeed, inhibition of α-amylase, a key enzyme that carries out the bulk of starch digestion, has been demonstrated for a range of bioactive compounds including anthocyanins; however, sample pigmentation often interferes with measurements, affecting colorimetric assay outcomes. Therefore, the present study compared the performance of a direct chromogenic assay, using 2-chloro-4 nitrophenyl α-D-maltotrioside (CNPG3) as a substrate, with the commonly used 3,5-dinitrosalicylic acid (DNS) assay. The direct chromogenic assay demonstrated a 5-10-fold higher sensitivity to determine α-amylase inhibition in various samples, including acarbose as a reference, pure anthocyanins, and anthocyanin-rich samples. The IC
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