Evidence map›Paper›PMID 37687030›Full record

ReviewMolecules (Basel, Switzerland)2023

Field-Flow Fractionation in Molecular Biology and Biotechnology.

Stefano Giordani, Valentina Marassi, Anna Placci, Andrea Zattoni, Barbara Roda, Pierluigi Reschiglian

Abstract readReview
In one paragraph

Review in Molecules (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Stefano GiordaniDepartment of Chemistry "Giacomo Ciamician", University of Bologna, 40126 Bologna, Italy.
Valentina MarassiDepartment of Chemistry "Giacomo Ciamician", University of Bologna, 40126 Bologna, Italy.ORCID 0000-0001-8742-3708
Anna PlacciDepartment of Chemistry "Giacomo Ciamician", University of Bologna, 40126 Bologna, Italy.
Andrea ZattoniDepartment of Chemistry "Giacomo Ciamician", University of Bologna, 40126 Bologna, Italy.ORCID 0000-0001-6971-5360
Barbara RodaDepartment of Chemistry "Giacomo Ciamician", University of Bologna, 40126 Bologna, Italy.ORCID 0000-0003-1060-2921
Pierluigi ReschiglianDepartment of Chemistry "Giacomo Ciamician", University of Bologna, 40126 Bologna, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension. These techniques cover an extremely wide dynamic range and are able to separate analytes in an interval between a few nm to 100 µm size-wise (over 15 orders of magnitude mass-wise). They are flexible in terms of mobile phase and can separate the analytes in native conditions, preserving their original structures/properties as much as possible. Molecular biology is the branch of biology that studies the molecular basis of biological activity, while biotechnology deals with the technological applications of biology. The areas where biotechnologies are required include industrial, agri-food, environmental, and pharmaceutical. Many species of biological interest belong to the operational range of FFF techniques, and their application to the analysis of such samples has steadily grown in the last 30 years. This work aims to summarize the main features, milestones, and results provided by the application of FFF in the field of molecular biology and biotechnology, with a focus on the years from 2000 to 2022. After a theoretical background overview of FFF and its methodologies, the results are reported based on the nature of the samples analyzed.

Indexed as

BiotechnologyFractionation, Field FlowFoodIndustryMolecular Biologyasymmetrical flow field-flow fractionation (AF4)bio-nanoparticlescell sortinglaser scatteringmolecular biologynative separationpharmaceuticsseparation science

Identifiers

PMID37687030
PMCPMC10488451

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.