Evidence map›Paper›PMID 37686023›Full record

ArticleInternational journal of molecular sciences2023

Revealing the SARS-CoV-2 Spike Protein and Specific Antibody Immune Complex Formation Mechanism for Precise Evaluation of Antibody Affinity.

Ieva Plikusiene, Vincentas Maciulis, Vilius Vertelis, Silvija Juciute, Saulius Balevicius, Arunas Ramanavicius, Julian Talbot, Almira Ramanaviciene

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ieva PlikusieneNanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.ORCID 0000-0002-7972-8891
Vincentas MaciulisNanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.ORCID 0000-0001-9552-8649
Vilius VertelisState Research Institute Center for Physical Sciences and Technology, Saulėtekio Ave. 3, LT-10257 Vilnius, Lithuania.
Silvija JuciuteNanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.
Saulius BaleviciusState Research Institute Center for Physical Sciences and Technology, Saulėtekio Ave. 3, LT-10257 Vilnius, Lithuania.
Arunas RamanaviciusNanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.ORCID 0000-0002-0885-3556
Julian TalbotLaboratoire de Physique Théorique de la Matière Condensée, Sorbonne Université, UMR 7600, 4 Place Jussieu, CEDEX 05, 75252 Paris, France.ORCID 0000-0003-1993-7883
Almira RamanavicieneNanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.ORCID 0000-0001-5864-0359

Funding

Lietuvos Mokslo Taryba 09.3.3-LMT-K-712-19-0106
6 · The paper itself

Abstract

The profound understanding and detailed evaluation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (SCoV2-S) protein and specific antibody interaction mechanism is of high importance in the development of immunosensors for COVID-19. In the present work, we studied a model system of immobilized SCoV2-S protein and specific monoclonal antibodies by molecular dynamics of immune complex formation in real time. We simultaneously applied spectroscopic ellipsometry and quartz crystal microbalance with dissipation to reveal the features and steps of the immune complex formation. We showed direct experimental evidence based on acoustic and optical measurements that the immune complex between covalently immobilized SCoV2-S and specific monoclonal antibodies is formed in two stages. Based on these findings it was demonstrated that applying a two-step binding mathematical model for kinetics analysis leads to a more precise determination of interaction rate constants than that determined by the 1:1 Langmuir binding model. Our investigation showed that the equilibrium dissociation constants (

Indexed as

Biosensing TechniquesCOVID-19Antibodies, MonoclonalAntibody AffinityAntigen-Antibody ComplexHumansImmunoassaySARS-CoV-2Spike Glycoprotein, CoronavirusAntibodies, MonoclonalAntigen-Antibody ComplexSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2affinity interactionantigen–antibody binding kineticsimmune complex formationQCM–DSARS-CoV-2spike protein

Identifiers

PMID37686023
PMCPMC10487573

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.