ArticleInternational journal of molecular sciences2023
Understanding the Mechanism of Light-Induced Age-Related Decrease in Melanin Concentration in Retinal Pigment Epithelium Cells.
Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Photooxidative molecular damage under blue light.Experimental & molecular medicine · 2026Review
- Sun Exposure and Behaviours in Saudi Arabia: A National Study of over Eleven Thousand Participants Utilising the Arabic Sun Exposure and Behaviour Inventory.Healthcare (Basel, Switzerland) · 2025Article
- Fluorescence Lifetime Imaging Microscopy Analysis of Isolated Melanosomes.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- Blue Light-Induced Accelerated Formation of Melanolipofuscin-Like Organelles in Japanese Quail RPE Cells: An Electron Microscopic Study.Investigative ophthalmology & visual science · 2024Article
- Retinal Pigment Epithelium Pigment Granules: Norms, Age Relations and Pathology.International journal of molecular sciences · 2024Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
It is known that during the process of aging, there is a significant decrease in the number of melanosomes in the retinal pigment epithelium (RPE) cells in the human eye. Melanosomes act as screening pigments in RPE cells and are fundamentally important for protection against the free radicals generated by light. A loss or change in the quality of melanin in melanosomes can lead to the development of senile pathologies and aggravation in the development of various retinal diseases. We have previously shown that the interaction between melanin melanosomes and superoxide radicals results in oxidative degradation with the formation of water-soluble fluorescent products. In the present study, we show, using fluorescence analysis, HPLC, and mass spectrometry, that visible light irradiation on melanolipofuscin granules isolated from RPE cells in the human eye results in the formation of water-soluble fluorescent products from oxidative degradation of melanin, which was in contrast to lipofuscin granules and melanosomes irradiation. The formation of these products occurs as a result of the oxidative degradation of melanin by superoxide radicals, which are generated by the lipofuscin part of the melanolipofuscin granule. We identified these products both in the composition of melanolipofuscin granules irradiated with visible light and in the composition of melanosomes that were not irradiated but were, instead, oxidized by superoxide radicals. In the melanolipofuscin granules irradiated by visible light, ions that could be associated with melanin oxidative degradation products were identified by applying the principal component analysis of the time-of-flight secondary ion mass spectrometry (ToF-SIMS) data. Degradation of the intact melanosomes by visible light is also possible; however, this requires significantly higher irradiation intensities than for melanolipofuscin granules. It is concluded that the decrease in the concentration of melanin in RPE cells in the human eye with age is due to its oxidative degradation by reactive oxygen species generated by lipofuscin, as part of the melanolipofuscin granules, under the action of light.
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