Evidence map›Paper›PMID 37684555›Full record

ArticleMolecular genetics and genomics : MGG2023

Characterization and diversity of CRISPR/Cas systems in Klebsiella oxytoca.

Jiaxue Zhao, Yanyan Xi, Jiangfeng Zhang, Yuefei Jin, Haiyan Yang, Guangcai Duan, Shuaiyin Chen, Jinzhao Long

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In one paragraph

Article in Molecular genetics and genomics : MGG, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
–field-weighted citation impact, top 72% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 0 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jiaxue ZhaoDepartment of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, Henan, China.
Yanyan XiDepartment of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, Henan, China.
Jiangfeng ZhangHenan Provincial People's Hospital, Zhengzhou, Henan, China.
Yuefei JinDepartment of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, Henan, China.
Haiyan YangDepartment of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, Henan, China.
Guangcai DuanDepartment of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, Henan, China.
Shuaiyin ChenDepartment of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, Henan, China. sychen@zzu.edu.cn.
Jinzhao LongDepartment of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, Henan, China. ljz069@zzu.edu.cn.ORCID http://orcid.org/0000-0002-0483-1122
Zhengzhou University · CNHenan Provincial People's Hospital · CN

Funding

China Postdoctoral Science Foundation 2022M712859National Science and Technology Specific Projects 2018ZX10301407
6 · The paper itself

Abstract

CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system is a crucial adaptive immune system for bacteria to resist foreign DNA infection. In this study, we investigated the prevalence and diversity of CRISPR/Cas systems in 175 Klebsiella oxytoca (K. oxytoca) strains. Specifically, 58.86% (103/175) of these strains possessed at least one confirmed CRISPR locus. Two CRISPR/Cas system types, I-F and IV-A3, were identified in 69 strains. Type I-F system was the most prevalent in this species, which correlated well with MLST. Differently, type IV-A3 system was randomly distributed. Moreover, the type IV-A3 system was separated into two subgroups, with subgroup-specific cas genes and repeat sequences. In addition, spacer origin analysis revealed that approximately one-fifth of type I-F spacers and one-third of type IV-A3 spacers had a significant match to MGEs. The phage tail tape measure protein and conjunctive transfer system protein were important targets of type I-F and IV-A3 systems in K. oxytoca, respectively. PAM sequences were inferred to be 5'-NCC-3' for type I-F, 5'-AAG-3' for subgroup IV-A3-a, and 5'-AAN-3' for subgroup IV-A3-b. Collectively, our findings will shed light on the prevalence, diversity, and functional effects of the CRISPR/Cas system in K. oxytoca.

Indexed as

CRISPR-Cas SystemsKlebsiella oxytocaMultilocus Sequence TypingCRISPR/Cas systemKlebsiella oxytocaMulti-locus sequence typingPhylogenetic analysesSpacers homology

Identifiers

PMID37684555
OpenAlexW4386533430

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.