Evidence map›Paper›PMID 37679637›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Applications of High-Throughput DNA Sequencing to Single-Domain Antibody Discovery and Engineering.

Michael J Lowden, Eric K Lei, Greg Hussack, Kevin A Henry

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. mAbs · 2026
    Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Michael J LowdenHuman Health Therapeutics Research Centre, National Research Council Canada, Ottawa, ON, Canada.
Eric K LeiHuman Health Therapeutics Research Centre, National Research Council Canada, Ottawa, ON, Canada.
Greg HussackHuman Health Therapeutics Research Centre, National Research Council Canada, Ottawa, ON, Canada.
Kevin A HenryHuman Health Therapeutics Research Centre, National Research Council Canada, Ottawa, ON, Canada. kevin.henry@nrc-cnrc.gc.ca.
National Research Council Canada · CANatural Sciences and Engineering Research Council of Canada · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Next-generation DNA sequencing (NGS) technologies have made it possible to interrogate antibody repertoires to unprecedented depths, typically via sequencing of cDNAs encoding immunoglobulin variable domains. In the absence of heavy-light chain pairing, the variable domains of heavy chain-only antibodies (HCAbs), referred to as single-domain antibodies (sdAbs), are uniquely amenable to NGS analyses. In this chapter, we provide simple and rapid protocols for producing and sequencing multiplexed immunoglobulin variable domain (V

Indexed as

Single-Domain AntibodiesB-LymphocytesCell Surface Display TechniquesHigh-Throughput Nucleotide SequencingImmunoglobulin Heavy ChainsTechnologyImmunoglobulin Heavy ChainsSingle-Domain AntibodiesAntibodyNext-generation DNA sequencingPhage displayProtein engineeringSingle-domain antibodyVHH

Identifiers

PMID37679637
OpenAlexW4386494396

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.