Evidence map›Paper›PMID 37679625›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Streptavidin-Coated Solid-Phase Extraction (SPE) Tips for Antibody Phage Display Biopanning.

Theam Soon Lim, Angela Chiew Wen Ch'ng, Brenda Pei Chui Song, Jing Yi Lai

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Theam Soon LimInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia. theamsoon@usm.my.
Angela Chiew Wen Ch'ngInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia.
Brenda Pei Chui SongInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia.
Jing Yi LaiInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phage display is a technique that allows the presentation of unique proteins on the surface of bacteriophages. The phage particles are usually screened via repetitive rounds of antigen-guided selection and phage amplification. The main advantage of this approach lies in the physical linkage between phenotype and genotype. This feature allows the isolation of single unique clones from a panning campaign consisting of a highly diverse population of clones. Due to the high-throughput nature of this technique, different approaches have been developed to assist phage display selections. One of which involves utilizing a streptavidin-coated solid-phase extraction (SPE) tip that is mounted to an electronically controlled motorized multichannel pipette. In this chapter, we will entail the procedures involved in the adaptation of a commercial SPE tip (MSIA™ streptavidin D.A.R.T's

Indexed as

BacteriophagesBioprospectingAntibodiesSolid Phase ExtractionStreptavidinAntibodiesStreptavidinBiopanningDisposable Automation Research Tips (D.A.R.T’s®)Mass spectrometry immunoassay (MSIA™)Monoclonal antibodiesPhage displaySolid-phase extraction (SPE)

Identifiers

PMID37679625

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.