Evidence map›Paper›PMID 37679621›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Construction of an Ultra-Large Phage Display Library by Kunkel Mutagenesis and Rolling Circle Amplification.

Renhua R Huang, Michael Kierny, Veronica Volgina, Makio Iwashima, Christina Miller, Brian K Kay

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Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Renhua R HuangMacroGenics, Rockville, MD, USA. huangr@macrogenics.com.
Michael KiernyAstraZeneca, Gaithersburg, MD, USA.
Veronica VolginaDepartment of Microbiology and Immunology, Loyola University Chicago, Maywood, IL, USA.
Makio IwashimaDepartment of Microbiology and Immunology, Loyola University Chicago, Maywood, IL, USA.
Christina MillerTango Biosciences, Chicago, IL, USA.
Brian K KayTango Biosciences, Chicago, IL, USA. bkay@tangobio.com.

Funding

Technology Development for Recombinant Affinity ReagentsU54DK093444 · NIDDK · UNIVERSITY OF ILLINOIS AT CHICAGO · PI KAY, BRIAN KENNETH · 2011 to 2013
$3.6M
Monocyte Regulation of Infant Immune ResponsesR01AI100129 · NIAID · LOYOLA UNIVERSITY CHICAGO · PI IWASHIMA, MAKIO, KNIGHT, KATHERINE L. · 2012 to 2016
$2.2M
Immunomodulatory properties of umbilical cord bloodR21AI100565 · NIAID · LOYOLA UNIVERSITY CHICAGO · PI IWASHIMA, MAKIO · 2013 to 2014
$398k
Building a Toolbox to Study the SARS-CoV-2 ProteomeR43AI157021 · NIAID · TANGO BIOSCIENCES, INC. · PI KAY, BRIAN KENNETH · 2021 to 2021
$222k
NIAID NIH HHS R01 AI100129NIAID NIH HHS R21 AI100565NIAID NIH HHS R43 AI157021NIDDK NIH HHS U54 DK093444
6 · The paper itself

Abstract

An important contributor to the successful generation of recombinant affinity reagents via phage display is a large and diverse library. We describe, herein, the application of Kunkel mutagenesis and rolling circle amplification (RCA) to the construction of a 1.1 × 10

Indexed as

BacteriophagesCOVID-19Gene LibraryHumansMutagenesisSARS-CoV-2Fibronectin type III (FN3) domainsKunkel mutagenesisLibrary constructionMonobodyRolling circle amplification (RCA)

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.