Evidence map›Paper›PMID 37679619›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Phagekines: Directed Evolution and Characterization of Functional Cytokines Displayed on Phages.

Gertrudis Rojas, Tania Carmenate, Gisela García-Pérez, Dayana Pérez-Martínez

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Gertrudis RojasCenter of Molecular Immunology, La Habana, Cuba. grojas@cim.sld.cu.
Tania CarmenateCenter of Molecular Immunology, La Habana, Cuba.
Gisela García-PérezCenter of Molecular Immunology, La Habana, Cuba.
Dayana Pérez-MartínezCenter of Molecular Immunology, La Habana, Cuba.
Center of Molecular Immunology (Cuba) · CU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The current chapter focuses on the use of filamentous phages to display and modify biologically active cytokines, with special emphasis on directed evolution of novel variants showing improved receptor binding. Cytokines are essential protein mediators involved in cell-to-cell communication. Their functional importance and the complexity of their interactions with multichain receptors make cytokine engineering a promising tool for the discovery and optimization of therapeutic molecules. Protocols used at the laboratory are illustrated through examples of manipulation of interleukin-2 and interleukin-6, two members of the family of alpha-helix-bundle cytokines playing pivotal roles in immunity and inflammation.

Indexed as

BacteriophagesCytokinesCell CommunicationHumansInflammationInterleukin-6CytokinesInterleukin-6CTLL-2 proliferation assayCytokine receptorELISAInterleukin-2Interleukin-6Kunkel mutagenesisLibraryPhage displaySTAT3 phosphorylationWestern blot

Identifiers

PMID37679619
OpenAlexW4386494541

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.