Evidence map›Paper›PMID 37676449›Full record

ArticleStress biology2022

Turnip mosaic virus manipulates DRM2 expression to regulate host CHH and CHG methylation for robust infection.

Xiaoyun Wu, Mengzhu Chai, Jiahui Liu, Xue Jiang, Yingshuai Yang, Yushuang Guo, Yong Li, Xiaofei Cheng

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stress biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it, 6 citations in OpenAlex.

  1. Pooled it
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Xiaoyun WuKey Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Mengzhu ChaiKey Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Jiahui LiuKey Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Xue JiangKey Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Yingshuai YangKey Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Yushuang GuoKey Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang, 550081, China.
Yong LiCollege of Life Science, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China. Yong@neau.edu.cn.
Xiaofei ChengKey Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, College of Agriculture, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China. xfcheng@neau.edu.cn.ORCID http://orcid.org/0000-0002-9771-9059
Northeast Agricultural University · CN

Funding

Academic Backbone Projects of Northeast Agricultural University 18XG04China National Tobacco Corporation 110202002010-JY-13National Natural Science Foundation of China 31860491National Natural Science Foundation of China 32022071Natural Science Foundation of Heilongjiang Municipal Science and Technology Commission LH2019C027
6 · The paper itself

Abstract

DNA methylation is an important epigenetic marker for the suppression of transposable elements (TEs) and the regulation of plant immunity. However, little is known how RNA viruses counter defense such antiviral machinery. In this study, the change of DNA methylation in turnip mosaic virus (TuMV)-infected cells was analyzed by whole genome bisulfite sequencing. Results showed that the total number of methylated sites of CHH and CHG increased in TuMV-infected cells, the majority of differentially methylated regions (DMRs) in the CHH and CHG contexts were associated with hypermethylation. Gene expression analysis showed that the expression of two methylases (DRM2 and CMT3) and three demethylases (ROS3, DML2, DML3) was significantly increased and decreased in TuMV-infected cells, respectively. Pathogenicity tests showed that the enhanced resistance to TuMV of the loss-of-function mutant of DRM2 is associated with unregulated expression of several defense-related genes. Finally, we found TuMV-encoded NIb, the viral RNA-dependent RNA polymerase, was able to induce the expression of DRM2. In conclusion, this study discovered that TuMV can modulate host DNA methylation by regulating the expression of DRM2 to promote virus infection.

Indexed as

Counter defenseDRM2HypermethylationRNA-directed DNA methylationTurnip mosaic virus

Identifiers

PMID37676449
PMCPMC10441925
OpenAlexW4289841433

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.