Evidence map›Paper›PMID 37676305›Full record

ArticleParasitology research2023

Functional characterization of CpADF, an actin depolymerizing factor protein in Cryptosporidium parvum.

Xiaotian Zhang, Luyang Wang, Ruiying Feng, Guanda Liang, Wenyan Hou, Yingying Zhang, Xiaoying Li, Longxian Zhang, Sumei Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Parasitology research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Xiaotian Zhang *College of Veterinary Medicine, Henan Agricultural University, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, People's Republic of China.
Luyang Wang *College of Veterinary Medicine, Henan Agricultural University, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, People's Republic of China.
Ruiying FengCollege of Veterinary Medicine, Henan Agricultural University, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, People's Republic of China.
Guanda LiangCollege of Veterinary Medicine, Henan Agricultural University, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, People's Republic of China.
Wenyan HouCollege of Veterinary Medicine, Henan Agricultural University, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, People's Republic of China.
Yingying ZhangCollege of Veterinary Medicine, Henan Agricultural University, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, People's Republic of China.
Xiaoying LiCollege of Veterinary Medicine, Henan Agricultural University, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, People's Republic of China.
Longxian ZhangCollege of Veterinary Medicine, Henan Agricultural University, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, People's Republic of China. zhanglx8999@henau.edu.cn.
Sumei ZhangCollege of Veterinary Medicine, Henan Agricultural University, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, People's Republic of China. smzhang2815@henau.edu.cn.
Ministry of Agriculture and Rural Affairs · CNHenan University of Engineering · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cryptosporidium is a highly pathogenic water and food-borne zoonotic parasitic protozoan that causes severe diarrhea in humans and animals. Apicomplexan parasites invade host cells via a unique motility process called gliding, which relies on the parasite's microfilaments. Actin depolymerizing factor (ADF) is a fibrous-actin (F-actin) and globular actin (G-actin) binding protein essential for regulating the turnover of microfilaments. However, the role of ADF in Cryptosporidium parvum (C. parvum) remains unknown. In this study, we preliminarily characterized the biological functions of ADF in C. parvum (CpADF). The CpADF was a 135-aa protein encoded by cgd5_2800 gene containing an ADF-H domain. The expression of cgd5_2800 gene peaked at 12 h post-infection, and the CpADF was located in the cytoplasm of oocysts, middle region of sporozoites, and cytoplasm of merozoites. Neutralization efficiency of anti-CpADF serum was approximately 41.30%. Actin sedimentation assay revealed that CpADF depolymerized but did not undergo cosedimentation with F-actin and its ability of F-actin depolymerization was pH independent. These results provide a basis for further investigation of the roles of CpADF in the invasion of C. parvum.

Indexed as

CryptosporidiosisCryptosporidiumCryptosporidium parvumActin Depolymerizing FactorsActinsAnimalsDestrinHumansMicrofilament ProteinsActin Depolymerizing FactorsActinsDestrinMicrofilament ProteinsActin depolymerizing factorCryptosporidium parvumDepolymerizationInvasion

Identifiers

PMID37676305
OpenAlexW4386504362

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.