ArticleFrontiers in immunology2023
Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of
Article in Frontiers in immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 19 citations in OpenAlex.
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- Novel Therapies for Prosthetic Joint Infections Caused by Methicillin-ResistantPathogens (Basel, Switzerland) · 2025Review
- Sequence variability of BamA and FadL candidate vaccinogens suggests divergent evolutionary paths ofJournal of bacteriology · 2025Article
- Site-directed antibodies targeting driver mutations of the KRAS protein.New biotechnology · 2025Article
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- Antibodies directed against extracellular loops of FadL orthologs disrupt outer membrane integrity and neutralize infectivity ofFrontiers in immunology · 2025Article
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- Syphilis vaccine development: Aligning vaccine design with manufacturing requirements.Human vaccines & immunotherapeutics · 2024Article
- Immunodominant extracellular loops of Treponema pallidum FadL outer membrane proteins elicit antibodies with opsonic and growth-inhibitory activities.PLoS pathogens · 2024Article
- Development and utilization ofbioRxiv : the preprint server for biology · 2024Article
- Article
- T-Cell Responses to Treponema pallidum Proteins in Blood and Skin to Advance Syphilis Vaccine Design: Learning From Nature.The Journal of infectious diseases · 2024Article
- Immunodominant extracellular loops ofbioRxiv : the preprint server for biology · 2024Article
- Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein.New biotechnology · 2024Article
- Article
- Benchmarking antibody discovery fidelity and reproducibility with an assay-locked residue fidelity index.BioTechniquesArticle
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 2 countries.
Funding
Abstract
Introduction: Syphilis, a sexually transmitted infection caused by the spirochete Methods: Three overlapping BamA ECL4 peptides and a two-stage, phage display strategy, termed "Epivolve" (for epitope evolution) were employed to generate single-chain variable fragments (scFvs). Additionally, antisera generated by immunizing mice and rabbits with BamA ECL4 displayed by a Results: Each of the five mAbs demonstrated reactivity by immunoblotting and ELISA to nanogram amounts of Discussion: Epivolve produced mAbs target subdominant opsonic epitopes in BamA ECL4, a top syphilis vaccine candidate. The murine opsonophagocytosis assay can serve as an alternative model to investigate the opsonic potential of vaccinogens. Detailed characterization of BamA ECL4-specific Abs provided a means to dissect Ab responses elicited by
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.