Evidence map›Paper›PMID 37674709›Full record

ArticleResearch square2023

Top-down mass spectrometry of native proteoforms and their complexes: A community study.

Frederik Lermyte, Tanja Habeck, Kyle Brown, Benjamin Des Soye, Carter Lantz, Mowei Zhou, Novera Alam, Md Amin Hossain, Wonhyeuk Jung, James Keener and 12 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

22 authors.

Frederik LermyteTechnische Universität Darmstadt.ORCID 0000-0001-7371-4475
Tanja HabeckTechnische Universität Darmstadt.
Kyle BrownUniversity of Wisconsin-Madison.ORCID 0000-0003-1255-9146
Benjamin Des SoyeNorthwestern University, Illinois, USA.
Carter LantzUniversity of California, Los Angeles.
Mowei ZhouPacific Northwest National Laboratory.
Novera AlamNortheastern University.
Md Amin HossainNortheastern University.
Wonhyeuk JungUniversity of California, Los Angeles.
James KeenerUniversity of Arizona.
Michael VolnyGenentech Inc.ORCID 0000-0001-7456-5408
Jesse WilsonPacific Northwest National Laboratory.
Yujia YingSun Yat-sen University.
Jeffrey AgarDepartment of Chemistry and Chemical Biology, Northeastern University.ORCID 0000-0003-2645-1873
Paul DanisEastwoods Consulting.
Ying GeUniversity of Wisconsin-Madison.ORCID 0000-0001-5211-6812
Neil KelleherNorthwestern University.ORCID 0000-0002-8815-3372
Huilin LiSun Yat-sen University.
Joseph LooUniversity of California, Los Angeles.ORCID 0000-0001-9989-1437
Michael MartyUniversity of Oxford.ORCID 0000-0001-8115-1772
Ljiljana Pasa-TolicPacific Northwest National Laboratory.ORCID 0000-0001-9853-5457
Wendy SandovalGenentech Inc.

Funding

UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
TR&D 7: Cell Specific ProteomicsP41GM108569 · NIGMS · NORTHWESTERN UNIVERSITY · PI KELLEHER, NEIL L · 2015 to 2024
$13.6M
Unravelling Membrane Protein-Lipid Interactions using Nanodiscs and Mass SpectrometryR35GM128624 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Michael T Marty · 2018 to 2026
$3.6M
UV Laser for Photodissociation-Mass Spectrometry of Proteins and Protein ComplexesR01GM103479 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LOO, JOSEPH A · 2012 to 2021
$3.2M
Ultra-High Resolution Mass Spectrometer for Biomedical ResearchS10RR028893 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LOO, JOSEPH A · 2010 to 2010
$2.7M
Ultra High Resolution Mass Spectrometer for Biomedical ResearchS10OD018475 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2015 to 2015
$2.0M
Advancing Mass Spectrometry Analyses of Proteins, Assemblies, and ProteoformsR35GM145286 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Joseph A Loo · 2022 to 2026
$1.9M
MASH Explorer, a Comprehensive Software Environment for Top-Down ProteomicsR01GM125085 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2018 to 2021
$1.2M
NCRR NIH HHS S10 RR028893NIGMS NIH HHS P41 GM108569NIGMS NIH HHS R01 GM103479NIGMS NIH HHS R01 GM125085NIGMS NIH HHS R35 GM128624NIGMS NIH HHS R35 GM145286NIGMS NIH HHS T32 GM007185NIH HHS S10 OD018475
6 · The paper itself

Abstract

The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spectrometry and top-down mass spectrometry are not yet well standardised, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics (CTDP) initiated a study to develop and test protocols for native mass spectrometry combined with top-down fragmentation of proteins and protein complexes across eleven instruments in nine laboratories. The outcomes are summarised in this report to provide robust benchmarks and a valuable entry point for the scientific community.

Identifiers

PMID37674709
PMCPMC10479449

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.