Evidence map›Paper›PMID 37674004›Full record

ArticleScientific reports2023

Surveillance of Vermont wildlife in 2021-2022 reveals no detected SARS-CoV-2 viral RNA.

Hannah W Despres, Margaret G Mills, Madaline M Schmidt, Jolene Gov, Yael Perez, Mars Jindrich, Allison M L Crawford, Arielle W Kohl, Elias Rosenblatt, Hannah C Kubinski and 46 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

56 authors at 5 institutions in 1 country.

Hannah W DespresDepartment of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405, USA.
Margaret G MillsVirology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Madaline M SchmidtDepartment of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405, USA.
Jolene GovVirology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Yael PerezVirology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Mars JindrichVirology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Allison M L CrawfordVirology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Arielle W KohlVirology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Elias RosenblattRubenstein School of Environment and Natural Resources, University of Vermont, 81 Carrigan Dr, Burlington, VT, 05405, USA.
Hannah C KubinskiDepartment of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405, USA.
Benjamin C SimmonsWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Miles C NippesWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Anne J GoldenbergWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Kristina E MurthaWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Samantha NicoloroWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Mia J HarrisWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Avery C FeeleyWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Taylor K GelinasWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Maeve K CroninWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Robert S FrederickWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Matthew ThomasWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Meaghan E JohnsonWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
James MurphyWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Elle B LenziniWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Peter A CarrWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Danielle H BergerWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Soham P MehtaWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Christopher J FloreaniWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Amelia C KovalWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Aleah L YoungWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Jess H FishWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Jack WallaceWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Ella ChaneyWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Grace UshayWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Rebecca S RossWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Erin M VostalWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Maya C ThisnerWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Kyliegh E GonetWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Owen C DeaneWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Kari R PelletiereWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Vegas C RockafellerWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Madeline WatermanWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Tyler W BarryWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Catriona C GoeringWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Sarah D ShipmanWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Allie C ShiersWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Claire E ReillyWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Alanna M DuffWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
Sarah L MadrugaWildlife and Fisheries Society, Wildlife Society Chapter, University of Vermont, Burlington, VT, 05405, USA.
David J ShirleyDepartment of Engineering, Faraday, Inc., Burlington, VT, 05405, USA.
Keith R JeromeVirology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Ailyn C Pérez-OsorioVirology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Alexander L GreningerVirology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Nick FortinFish and Wildlife Department, Vermont Agency of Natural Resources, Rutland, VT, 05701, USA.
Brittany A MosherRubenstein School of Environment and Natural Resources, University of Vermont, 81 Carrigan Dr, Burlington, VT, 05405, USA. Brittany.mosher@uvm.edu.
Emily A BruceDepartment of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405, USA. Emily.bruce@med.uvm.edu.
University of Vermont · USThe Wildlife Society · USUniversity of Washington · USFaraday Technology (United States) · USVermont Fish & Wildlife Department · US

Funding

Vermont Immunology/Infectious Diseases CenterP30GM118228 · NIGMS · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI BUDD, RALPH C · 2016 to 2021
$6.8M
NIGMS NIH HHS P30 GM118228
6 · The paper itself

Abstract

Previous studies have documented natural infections of SARS-CoV-2 in various domestic and wild animals. More recently, studies have been published noting the susceptibility of members of the Cervidae family, and infections in both wild and captive cervid populations. In this study, we investigated the presence of SARS-CoV-2 in mammalian wildlife within the state of Vermont. 739 nasal or throat samples were collected from wildlife throughout the state during the 2021 and 2022 harvest season. Data was collected from red and gray foxes (Vulpes vulples and Urocyon cineroargentus, respectively), fishers (Martes pennati), river otters (Lutra canadensis), coyotes (Canis lantrans), bobcats (Lynx rufus rufus), black bears (Ursus americanus), and white-tailed deer (Odocoileus virginianus). Samples were tested for the presence of SARS-CoV-2 via quantitative RT-qPCR using the CDC N1/N2 primer set and/or the WHO-E gene primer set. Surprisingly, we initially detected a number of N1 and/or N2 positive samples with high cycle threshold values, though after conducting environmental swabbing of the laboratory and verifying with a second independent primer set (WHO-E) and PCR without reverse transcriptase, we showed that these were false positives due to plasmid contamination from a construct expressing the N gene in the general laboratory environment. Our final results indicate that no sampled wildlife were positive for SARS-CoV-2 RNA, and highlight the importance of physically separate locations for the processing of samples for surveillance and experiments that require the use of plasmid DNA containing the target RNA sequence. These negative findings are surprising, given that most published North America studies have found SARS-CoV-2 within their deer populations. The absence of SARS-CoV-2 RNA in populations sampled here may provide insights in to the various environmental and anthropogenic factors that reduce spillover and spread in North American's wildlife populations.

Indexed as

COVID-19CoyotesDeerLynxOttersAnimalsAnimals, WildFoxesRNA, ViralSARS-CoV-2VermontRNA, Viral

Identifiers

PMID37674004
PMCPMC10482933
OpenAlexW4386477684

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.