ArticleCell reports methods2023
Non-invasive monitoring of microbiota and host metabolism using secondary electrospray ionization-mass spectrometry.
Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Gut microbiota restricts intestinal lipid uptake via modulation of bile phosphatidylcholine metabolism in mice.Nature microbiology · 2026Article
- Emergent spatial structure in the gut microbiota is driven by bacterial growth and gut contractions.PLoS biology · 2026Article
- Diagnosing and engineering gut microbiomes.EMBO molecular medicine · 2024Review
- An engineered bacterial symbiont allows noninvasive biosensing of the honey bee gut environment.PLoS biology · 2024Article
- Online breath analysis with SESI/HRMS for metabolic signatures in children with allergic asthma.Frontiers in molecular biosciences · 2023Article
- Practical Applications of Secondary/Extractive Electrospray Ionization (SESI): A Versatile Tool for Real-Time Chemical Analysis.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The metabolic "handshake" between the microbiota and its mammalian host is a complex, dynamic process with major influences on health. Dissecting the interaction between microbial species and metabolites found in host tissues has been a challenge due to the requirement for invasive sampling. Here, we demonstrate that secondary electrospray ionization-mass spectrometry (SESI-MS) can be used to non-invasively monitor metabolic activity of the intestinal microbiome of a live, awake mouse. By comparing the headspace metabolome of individual gut bacterial culture with the "volatilome" (metabolites released to the atmosphere) of gnotobiotic mice, we demonstrate that the volatilome is characteristic of the dominant colonizing bacteria. Combining SESI-MS with feeding heavy-isotope-labeled microbiota-accessible sugars reveals the presence of microbial cross-feeding within the animal intestine. The microbiota is, therefore, a major contributor to the volatilome of a living animal, and it is possible to capture inter-species interaction within the gut microbiota using volatilome monitoring.
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Registered trials
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