Evidence map›Paper›PMID 37668906›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ).

Emily Montal, Shruthy Suresh, Yilun Ma, Mohita M Tagore, Richard M White

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Zebrafish: A Versatile and Powerful Model for Biomedical Research.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Emily MontalCancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Shruthy SureshCancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Yilun MaCancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Mohita M TagoreCancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Richard M WhiteCancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA. whiter@mskcc.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transgenic expression of genes is a mainstay of cancer modeling in zebrafish. Traditional transgenic techniques rely upon injection into one-cell embryos, but ideally these transgenes would be expressed only in adult somatic tissues. We provide a method to model cancer in adult zebrafish in which transgenes can be expressed via electroporation. Using melanoma as an example, we demonstrate the feasibility of expressing oncogenes such as BRAFV600E as well as CRISPR/Cas9 inactivation of tumor suppressors such as PTEN. These approaches can be performed in any genetic background such as existing fluorophore reporter lines or the casper line. These methods can readily be extended to other cell types allowing for rapid adult modeling of cancer in zebrafish.

Indexed as

NeoplasmsZebrafishAnimalsAnimals, Genetically ModifiedElectroporationElectroporation TherapiesTransgenesCancer modelingCRISPR/Cas9ElectroporationMelanomaOncogenesSomatic transgenesisTumor suppressors

Identifiers

PMID37668906

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.