Evidence map›Paper›PMID 37668902›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Delivering Traumatic Brain Injury to Larval Zebrafish.

Taylor Gill, Laszlo F Locskai, Alexander H Burton, Hadeel Alyenbaawi, Travis Wheeler, Edward A Burton, W Ted Allison

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
6.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 3 countries.

Taylor Gill *Centre for Prions & Protein Folding Disease, University of Alberta, Edmonton, AB, Canada.
Laszlo F Locskai *Centre for Prions & Protein Folding Disease, University of Alberta, Edmonton, AB, Canada.
Alexander H BurtonDepartments of Chemical and Biomedical Engineering, College of Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Hadeel AlyenbaawiDepartment of Medical Laboratories, Majmaah University, Majmaah, Saudi Arabia.
Travis WheelerDepartment of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA.
Edward A BurtonDepartment of Neurology, University of Pittsburgh, Pittsburgh, PA, USA.
W Ted AllisonCentre for Prions & Protein Folding Disease, University of Alberta, Edmonton, AB, Canada. ted.allison@ualberta.ca.
University of Alberta · CAUniversity of Pittsburgh · USCarnegie Mellon University · USMajmaah University · SA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a straightforward, scalable method for administering traumatic brain injury (TBI) to zebrafish larvae. The pathological outcomes appear generalizable for all TBI types, but perhaps most closely model closed-skull, diffuse lesion (blast injury) neurotrauma. The injury is delivered by dropping a weight onto the plunger of a fluid-filled syringe containing zebrafish larvae. This model is easy to implement, cost-effective, and provides a high-throughput system that induces brain injury in many larvae at once. Unique to vertebrate TBI models, this method can be used to deliver TBI without anesthetic or other metabolic agents. The methods simulate the main aspects of traumatic brain injury in humans, providing a preclinical model to study the consequences of this prevalent injury type and a way to explore early interventions that may ameliorate subsequent neurodegeneration. We also describe a convenient method for executing pressure measurements to calibrate and validate this method. When used in concert with the genetic tools readily available in zebrafish, this model of traumatic brain injury offers opportunities to examine many mechanisms and outcomes induced by traumatic brain injury. For example, genetically encoded fluorescent reporters have been implemented with this system to measure protein misfolding and neural activity via optogenetics.

Indexed as

Blast InjuriesBrain InjuriesBrain Injuries, TraumaticAnimalsHumansLarvaZebrafishAnimal modelBlast injuryClosed head injuryConcussionNeurotraumaPressure transducer

Identifiers

PMID37668902
OpenAlexW4386437965

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.