Evidence map›Paper›PMID 37666630›Full record

ArticleMethods in enzymology2023

Cell-based transport assay to study kinetics and substrate specificity of human ZIPs.

Yuhan Jiang, Dexin Sui, Jian Hu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 79% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Yuhan JiangDepartment of Chemistry, Michigan State University, East Lansing, MI, United States.
Dexin SuiDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, United States.
Jian HuDepartment of Chemistry, Michigan State University, East Lansing, MI, United States; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, United States. Electronic address: hujian1@msu.edu.
Michigan State University · US

Funding

Transport, substrate specificity and regulation mechanisms of the ZIP transition metal transportersR35GM140931 · NIGMS · MICHIGAN STATE UNIVERSITY · PI HU, JIAN · 2021 to 2025
$1.7M
Zinc enrichment/zinc sensing mechanism through the extracellular domain of ZIP4R01GM115373 · NIGMS · MICHIGAN STATE UNIVERSITY · PI HU, JIAN · 2015 to 2019
$1.5M
Structural and Mechanistic Characterization of the ZIP Metal TransportersR01GM129004 · NIGMS · MICHIGAN STATE UNIVERSITY · PI HU, JIAN · 2018 to 2020
$593k
NIGMS NIH HHS R01 GM115373NIGMS NIH HHS R01 GM129004NIGMS NIH HHS R35 GM140931
6 · The paper itself

Abstract

Kinetic study of human ZIPs is crucial for understanding the transport mechanism and the molecular basis of substrate specificity. In this chapter, we describe the detailed experimental procedures for functional studies of two human ZIPs, including the zinc-preferring ZIP4 and the multi-metal transporter ZIP8, by using the cell-based transport assays. Kinetic study of ZIP4 is elaborated in the first section; in the second section, comparison of ZIP4 and ZIP8 in terms of the zinc/cadmium selectivity is performed by using an internal competition assay adapted from the established cell-based approach. The protocols provided in this chapter will facilitate mechanistic and engineering studies of the ZIPs.

Indexed as

Biological AssayMembrane Transport ProteinsHumansKineticsSubstrate SpecificityZincMembrane Transport ProteinsZincCadmiumCell-based assaySubstrate promiscuitySubstrate specificityZincZIP4ZIP8

Identifiers

PMID37666630
PMCPMC10999280
OpenAlexW4381747318

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.