ArticleDevelopment (Cambridge, England)2023
An inducible germ cell ablation chicken model for high-grade germline chimeras.
Article in Development (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 17 citations in OpenAlex.
- Review
- Retinoic Acid Signalling Regulates Zebrafish Tooth Germ Repair Following Injury.Cell proliferation · 2026Article
- NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis.Poultry science · 2026Article
- Transcriptome Comparison Between the Cultured and In Vivo Chick Primordial Germ Cells by SMART-Seq-Based Single-Cell RNA Sequencing.Development, growth & differentiation · 2026Article
- Manipulating primordial germ cells from chicken and other bird species.The Journal of reproduction and development · 2026Review
- Are we truly helping those in need: A comprehensive assessment of semen technologies in wild bird species.Biodiversity and conservation · 2026Article
- Eye pigmentation-based in-ovo chicken sexing via precision breeding.Frontiers in bioengineering and biotechnology · 2026Article
- In Vitro Culture of Avian Primordial Germ Cells: Established Methods and Future Directions.Biology · 2025Review
- Genome Editing in the Chicken: From PGC-Mediated Germline Transmission to Advanced Applications.International journal of molecular sciences · 2025Review
- Spatiotemporal control of cell ablation using Ronidazole with Nitroreductase in Drosophila.Developmental biology · 2025Article
- Labeling and sorting of avian primordial germ cells utilizing Lycopersicon Esculentum lectin.Development, growth & differentiation · 2024Article
- Successful formation of sperm cells from transplanted primordial germ cells in sterile interspecific avian recipients.Scientific reports · 2024Article
- Cas12a and MAD7, genome editing tools for breeding.Breeding science · 2024Article
- Genetically modified chickens as bioreactors for protein-based drugs.Frontiers in genome editing · 2024Review
- Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells.Frontiers in genome editing · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chicken embryos are a powerful and widely used animal model in developmental biology studies. Since the development of CRISPR technology, gene-edited chickens have been generated by transferring primordial germ cells (PGCs) into recipients after genetic modifications. However, low inheritance caused by competition between host germ cells and the transferred cells is a common complication and greatly reduces production efficiency. Here, we generated a gene-edited chicken, in which germ cells can be ablated in a drug-dependent manner, as recipients for gene-edited PGC transfer. We used the nitroreductase/metronidazole (NTR/Mtz) system for cell ablation, in which nitroreductase produces cytotoxic alkylating agents from administered metronidazole, causing cell apoptosis. The chicken Vasa homolog (CVH) gene locus was used to drive the expression of the nitroreductase gene in a germ cell-specific manner. In addition, a fluorescent protein gene, mCherry, was also placed in the CVH locus to visualize the PGCs. We named this system 'germ cell-specific autonomous removal induction' (gSAMURAI). gSAMURAI chickens will be an ideal recipient to produce offspring derived from transplanted exogenous germ cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.