ArticleFrontiers in veterinary science2023
Multiplex digital PCR: a superior technique to qPCR for the simultaneous detection of duck Tembusu virus, duck circovirus, and new duck reovirus.
Article in Frontiers in veterinary science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 18 citations in OpenAlex.
- Development and application of a quadruple RT-qPCR assay for simultaneous detection of GPV, GPMV, NGRV, and TMUV.Poultry science · 2026Article
- Development of a Triplex TaqMan Real-Time PCR Assay for Simultaneous Detection of Duck Hepatitis B Virus, Duck Adenovirus Type 3, andVeterinary sciences · 2026Article
- Development and validation of a crystal digital PCR assay for duck Tembusu virus detection and vaccine monitoring.Poultry science · 2026Article
- Development and comparison of duplex crystal digital PCR and qPCR assays for the detection of Muscovy duck parvovirus and goose parvovirus.Poultry science · 2026Article
- Development of an RT-ERA-CRISPR/Cas12a assay for duck Tembusu virus.Poultry science · 2026Article
- A novel five-plex digital PCR assay for the simultaneous detection of murine pathogens: Sendai virus, reovirus, mouse parvoviruses, pneumonia virus of mice, and mouse hepatitis virus.Microbiology spectrum · 2026Article
- Navigating the Complexity: Advancing Diagnostic Strategies for Avian Reovirus in Chinese Poultry.Animals : an open access journal from MDPI · 2026Review
- Overview of waterfowl circovirus: research progress and future perspectives.Frontiers in veterinary science · 2026Review
- TaqMan-probe-based multiplex real-time qPCR assay for simultaneous detection of Duck Tembusu virus, Novel duck reovirus, and Duck circovirus.Frontiers in microbiology · 2026Article
- Epidemiological investigation and whole-genome genetic characterization of duck circovirus in Shandong Province of eastern China.Frontiers in veterinary science · 2026Article
- Development of a TaqMan-based triplex qPCR assay for the simultaneous detection of Muscovy reovirus, novel duck reovirus, andFrontiers in veterinary science · 2026Article
- Establishment of a Visual Gene Chip Method for the Simultaneous Detection of Seven Waterfowl Virus Pathogens.Viruses · 2025Article
- Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.Animals : an open access journal from MDPI · 2024Article
- One-Step Multiplex Real-Time Fluorescent Quantitative Reverse Transcription PCR for Simultaneous Detection of Four Waterfowl Viruses.Microorganisms · 2024Article
- Review
- Development and application of quadruplex real time quantitative PCR method for differentiation of Muscovy duck parvovirus, Goose parvovirus, Duck circovirus, and Duck adenovirus 3.Frontiers in cellular and infection microbiology · 2024Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Duck Tembusu virus (DTMUV), duck circovirus (DuCV), and new duck reovirus (NDRV) have seriously hindered the development of the poultry industry in China. To detect the three pathogens simultaneously, a multiplex digital PCR (dPCR) was developed and compared with multiplex qPCR in this study. The multiplex dPCR was able to specifically detect DTMUV, DuCV, and NDRV but not amplify Muscovy duck reovirus (MDRV), Muscovy duck parvovirus (MDPV), goose parvovirus (GPV), H4 avian influenza virus (H4 AIV), H6 avian influenza virus (H6 AIV), and Newcastle disease virus (NDV). The standard curves showed excellent linearity in multiplex dPCR and qPCR and were positively correlated. The sensitivity results showed that the lowest detection limit of multiplex dPCR was 1.3 copies/μL, which was 10 times higher than that of multiplex qPCR. The reproducibility results showed that the intra- and interassay coefficients of variation were 0.06-1.94%. A total of 173 clinical samples were tested to assess the usefulness of the method; the positive detection rates for DTMUV, DuCV, and NDRV were 18.5, 29.5, and 14.5%, respectively, which were approximately 4% higher than those of multiplex qPCR, and the kappa values for the clinical detection results of multiplex dPCR and qPCR were 0.85, 0.89, and 0.86, indicating that the two methods were in excellent agreement.
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