Evidence map›Paper›PMID 37662340›Full record

ArticlebioRxiv : the preprint server for biology2023

Deep screening of proximal and distal splicing-regulatory elements in a native sequence context.

Yocelyn Recinos, Dmytro Ustianenko, Yow-Tyng Yeh, Xiaojian Wang, Martin Jacko, Lekha V Yesantharao, Qiyang Wu, Chaolin Zhang

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Yocelyn RecinosDepartment of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.
Dmytro UstianenkoDepartment of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.
Yow-Tyng YehDepartment of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.
Xiaojian WangDepartment of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.
Martin JackoDepartment of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.
Lekha V YesantharaoDepartment of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.
Qiyang WuDepartment of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.
Chaolin ZhangDepartment of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.
Columbia University · USFlagship Pioneering (United States) · USJohns Hopkins University · USPrescient Surgical (United States) · US

Funding

Complexity and evolution of splicing-regulatory networksR35GM145279 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Chaolin Zhang · 2022 to 2026
$4.6M
Mapping proximal and distal splicing-regulatory elementsR01HG012359 · NHGRI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Chaolin Zhang · 2023 to 2026
$2.4M
BD Biosciences InfluxS10OD020056 · OD · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI SNOECK, HANS-WILLEM E · 2015 to 2015
$600k
Mapping proximal and distal splicing-regulatory elementsR56HG012359 · NHGRI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI ZHANG, CHAOLIN · 2022 to 2022
$569k
Becton Dickinson LSR II Analytical Flow CytometerS10RR027050 · NCRR · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI CLYNES, RAPHAEL A. · 2011 to 2011
$413k
NCRR NIH HHS S10 RR027050NHGRI NIH HHS R01 HG012359NHGRI NIH HHS R56 HG012359NIGMS NIH HHS R35 GM145279NIH HHS S10 OD020056
6 · The paper itself

Abstract

Pre-mRNA splicing, a key process in gene expression, can be therapeutically modulated using various drug modalities, including antisense oligonucleotides (ASOs). However, determining promising targets is impeded by the challenge of systematically mapping splicing-regulatory elements (SREs) in their native sequence context. Here, we use the catalytically dead CRISPR-

Indexed as

alternative splicingantisense oligonucleotides (ASO)dCas13d/guide RNAhigh-throughput screenSMN2splicing-regulatory elements (SREs)

Identifiers

PMID37662340
PMCPMC10473672
OpenAlexW4386050772

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.