Evidence map›Paper›PMID 37662218›Full record

ArticlebioRxiv : the preprint server for biology2023

Supporting materials: Endothelial cells differentiated from patient dermal fibroblast-derived induced pluripotent stem cells resemble vascular malformations of Port Wine Birthmark.

Vi Nguyen, Chao Gao, Marcelo L Hochman, Jacob Kravitz, Elliott H Chen, Harold I Friedman, Camilla F Wenceslau, Dongbao Chen, Yunguan Wang, J Stuart Nelson and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 6 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Vi NguyenDepartment of Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, South Carolina 29209, USA.
Chao GaoDepartment of Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, South Carolina 29209, USA.
Marcelo L HochmanThe Facial Surgery Center and the Hemangioma & Malformation Treatment Center, Charleston, South Carolina 29425, USA.
Jacob KravitzDepartment of Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, South Carolina 29209, USA.
Elliott H ChenDivision of Plastic Surgery, School of Medicine, University of South Carolina, Columbia, South Carolina 29203, USA.
Harold I FriedmanDivision of Plastic Surgery, School of Medicine, University of South Carolina, Columbia, South Carolina 29203, USA.
Camilla F WenceslauDepartment of Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, South Carolina 29209, USA.
Dongbao ChenDepartment of Obstetrics and Gynecology, University of California, Irvine, Irvine, California 92617, USA.
Yunguan WangDepartment of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA.
J Stuart NelsonDepartments of Surgery and Biomedical Engineering, Beckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, California 92617, USA.
Anil G JeggaDepartment of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA.
Wenbin TanDepartment of Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, South Carolina 29209, USA.
University of South Carolina · USCincinnati Children's Hospital Medical Center · USSouth Carolina Department of Health and Environmental Control · USUniversity of California, Irvine · USMedical University of South Carolina · US

Funding

Vascular Pathogenesis of Port Wine StainR01AR073172 · NIAMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI TAN, WENBIN · 2018 to 2022
$1.5M
Generation and Validation of Disease Models for Port-Wine BirthmarksR21AR083066 · NIAMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI TAN, WENBIN · 2023 to 2024
$356k
NIAMS NIH HHS R01 AR073172NIAMS NIH HHS R21 AR083066
6 · The paper itself

Abstract

Background: Port wine birthmark (PWB) is a congenital vascular malformation resulting from developmentally defective endothelial cells (ECs). Developing clinically relevant disease models for PWB studies is currently an unmet need. Objective: Our study aims to generate PWB-derived induced pluripotent stem cells (iPSCs) and iPSC-derived ECs that preserve disease-related phenotypes. Methods: PWB iPSCs were generated by reprogramming lesional dermal fibroblasts and differentiated into ECs. RNA-seq was performed to identify differentially expressed genes (DEGs) and enriched pathways. The functional phenotypes of iPSC-derived ECs were characterized by capillary-like structure (CLS) formation Results: Human PWB and control iPSC lines were generated through reprogramming of dermal fibroblasts by introducing the "Yamanaka factors" (Oct3/4, Sox2, Klf4, c-Myc) into them; the iPSCs were successfully differentiated into ECs. These iPSCs and their derived ECs were validated by expression of a series of stem cell and EC biomarkers, respectively. PWB iPSC-derived ECs showed impaired CLS Conclusions: PWB iPSC-derived ECs render a novel and clinically-relevant disease model by retaining pathological phenotypes. Our data demonstrate multiple pathways, such as Hippo and Wnt, NF-kappa B, TNF, MAPK, and cholesterol metabolism, are dysregulated, which may contribute to the development of differentiation-defective ECs in PWB. Bulleted statements:

Identifiers

PMID37662218
PMCPMC10473620
OpenAlexW4382931189

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.