Evidence map›Paper›PMID 37662163›Full record

ArticleFrontiers in plant science2023

Measuring calcium content in plants using NEXAFS spectroscopy.

Sintu Rongpipi, William J Barnes, Oskar Siemianowski, Joshua T Del Mundo, Cheng Wang, Guillaume Freychet, Mikhail Zhernenkov, Charles T Anderson, Esther W Gomez, Enrique D Gomez

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Sintu RongpipiDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, United States.
William J BarnesDepartment of Biology, The Pennsylvania State University, University Park, PA, United States.
Oskar SiemianowskiDepartment of Biology, The Pennsylvania State University, University Park, PA, United States.
Joshua T Del MundoDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, United States.
Cheng WangAdvanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
Guillaume FreychetNational Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY, United States.
Mikhail ZhernenkovNational Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY, United States.
Charles T AndersonDepartment of Biology, The Pennsylvania State University, University Park, PA, United States.
Esther W GomezDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, United States.
Enrique D GomezDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, United States.
Pennsylvania State University · USBrookhaven National Laboratory · USLawrence Berkeley National Laboratory · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Calcium is important for the growth and development of plants. It serves crucial functions in cell wall and cell membrane structure and serves as a secondary messenger in signaling pathways relevant to nutrient and immunity responses. Thus, measuring calcium levels in plants is important for studies of plant biology and for technology development in food, agriculture, energy, and forest industries. Often, calcium in plants has been measured through techniques such as atomic absorption spectrophotometry (AAS), inductively coupled plasma-mass spectrometry (ICP-MS), and electrophysiology. These techniques, however, require large sample sizes, chemical extraction of samples or have limited spatial resolution. Here, we used near-edge X-ray absorption fine structure (NEXAFS) spectroscopy at the calcium L- and K-edges to measure the calcium to carbon mass ratio with spatial resolution in plant samples without requiring chemical extraction or large sample sizes. We demonstrate that the integrated absorbance at the calcium L-edge and the edge jump in the fluorescence yield at the calcium K-edge can be used to quantify the calcium content as the calcium mass fraction, and validate this approach with onion epidermal peels and ICP-MS. We also used NEXAFS to estimate the calcium mass ratio in hypocotyls of a model plant,

Indexed as

Arabidopsis hypocotylfluorescence yield NEXAFSinductively coupled plasma mass spectrometryonion epidermisprimary cell walltransmission NEXAFS

Identifiers

PMID37662163
PMCPMC10468975
OpenAlexW4385994469

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.