Evidence map›Paper›PMID 37662148›Full record

ArticleFrontiers in plant science2023

Development and application of Single Primer Enrichment Technology (SPET) SNP assay for population genomics analysis and candidate gene discovery in lettuce.

Pasquale Tripodi, Massimiliano Beretta, Damien Peltier, Ilias Kalfas, Christos Vasilikiotis, Anthony Laidet, Gael Briand, Charlotte Aichholz, Tizian Zollinger, Rob van Treuren and 2 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 5 countries.

Pasquale TripodiCouncil for Agricultural Research and Economics (CREA), Research Centre for Vegetable and Ornamental Crops, Pontecagnano Faiano, SA, Italy.
Massimiliano BerettaISI Sementi SpA, Fidenza (PR), Italy.
Damien PeltierLimagrain - Vilmorin-Mikado, La Ménitré, France.
Ilias KalfasAmerican Farm School, Thessaloniki, Greece.
Christos VasilikiotisPerrotis College, American Farm School, Thessaloniki, Greece.
Anthony LaidetGautier Semences Route d'Avignon 13630, Eyragues, France.
Gael BriandGautier Semences Route d'Avignon 13630, Eyragues, France.
Charlotte AichholzSativa Rheinau AG, Rheinau, Switzerland.
Tizian ZollingerZollinger Conseilles Sarl, Les Evouettes, Switzerland.
Rob van TreurenCentre for Genetic Resources, the Netherlands (CGN), Wageningen University and Research, Wageningen, Netherlands.
Davide ScaglioneIGA Technology Services Srl, Udine, Italy.
Sandra GoritschnigEuropean Cooperative Programme for Plant Genetic Resources (ECPGR) Secretariat c/o Alliance of Bioversity International and CIAT, Rome, Italy.
American Farm School · GRAlliance Bioversity International - CIAT · ITIstituto di Genomica Applicata · ITLimagrain (France) · FRResearch Centre for Cereal and Industrial Crops · ITRheinmetall (Switzerland) · CHWageningen University & Research · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single primer enrichment technology (SPET) is a novel high-throughput genotyping method based on short-read sequencing of specific genomic regions harboring polymorphisms. SPET provides an efficient and reproducible method for genotyping target loci, overcoming the limits associated with other reduced representation library sequencing methods that are based on a random sampling of genomic loci. The possibility to sequence regions surrounding a target SNP allows the discovery of thousands of closely linked, novel SNPs. In this work, we report the design and application of the first SPET panel in lettuce, consisting of 41,547 probes spanning the whole genome and designed to target both coding (~96%) and intergenic (~4%) regions. A total of 81,531 SNPs were surveyed in 160 lettuce accessions originating from a total of 10 countries in Europe, America, and Asia and representing 10 horticultural types. Model ancestry population structure clearly separated the cultivated accessions (

Indexed as

candidate genesgenomic diversityGWAShigh-throughput genotypinglettucephenotypingSPET

Identifiers

PMID37662148
PMCPMC10471991
OpenAlexW4385984963

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.