Evidence map›Paper›PMID 37658990›Full record

ArticleMarine biotechnology (New York, N.Y.)2023

Cold Stress Induces Apoptosis in Silver Pomfret via DUSP-JNK Pathway.

Jiabao Hu, Man Zhang, Kaiheng Yan, Youyi Zhang, Yaya Li, Jiajie Zhu, Guanlin Wang, Xiangbing Wang, Yuanbo Li, Xiang Huang and 6 more

Abstract read
PubMed Publisher
In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 14 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 1 institution in 1 country.

Jiabao Hu *College of Marine Sciences, Ningbo University, Ningbo, China.
Man Zhang *College of Marine Sciences, Ningbo University, Ningbo, China.
Kaiheng YanCollege of Marine Sciences, Ningbo University, Ningbo, China.
Youyi ZhangCollege of Marine Sciences, Ningbo University, Ningbo, China.
Yaya LiCollege of Marine Sciences, Ningbo University, Ningbo, China.
Jiajie ZhuCollege of Marine Sciences, Ningbo University, Ningbo, China.
Guanlin WangCollege of Marine Sciences, Ningbo University, Ningbo, China.
Xiangbing WangCollege of Marine Sciences, Ningbo University, Ningbo, China.
Yuanbo LiCollege of Marine Sciences, Ningbo University, Ningbo, China.
Xiang HuangCollege of Marine Sciences, Ningbo University, Ningbo, China.
Jie TangCollege of Marine Sciences, Ningbo University, Ningbo, China.
Rongyue ZhengSchool of Civil & Environmental Engineering and Geography Science, Ningbo University, Ningbo, China.
Shanliang XuCollege of Marine Sciences, Ningbo University, Ningbo, China.
Danli WangCollege of Marine Sciences, Ningbo University, Ningbo, China.
Yajun WangCollege of Marine Sciences, Ningbo University, Ningbo, China. wangyajun@nbu.edu.cn.
Xiaojun YanCollege of Marine Sciences, Ningbo University, Ningbo, China.
Ningbo University · CN

Funding

Natural Science Foundation of Zhejiang Province LY18C190008 and LY18C1900013Science and Technology Innovation 2025 Major Project of Ningbo 2021Z003Zhejiang Major Science Project 2019C02059
6 · The paper itself

Abstract

We cultured silver pomfret for 20 days, decreasing water temperature from 18 to 8 ℃, and sampled muscle every 5 days. Muscle fiber degeneration and apoptosis began to increase at 13 ℃ detected by HE and TUNEL staining. Further analysis of transcriptome revealed that several apoptosis-related pathways were highly enriched by differentially expressed genes (DEGs). We analyzed 10 DEGs from these pathways by RT-qPCR during the temperature-decreasing process. JNK1, PIDD, CytC, Casp 3, and GADD45 were up-regulated after 15 and 20 days, while DUSP3, JNK2, and PARP genes were down-regulated after 15 and 20 days. DUSP5 was up-regulated from 10 to 20 days, and C-JUN was up-regulated after 20 days. We analyzed apoptosis in PaM cells under different temperatures (26 ℃, 23 ℃, 20 ℃, 17 ℃, and 14 ℃). The cell viability significantly declined from 14 to 20 ℃; the TUNEL and IHC results showed that the apoptosis signal increased with the temperature dropping, especially in 17 ℃ and 14 ℃; DUSP5, JNK1, CytC, C-JUN, Casp 3, and GADD45 were up-regulated at 17 ℃ and 14 ℃, and PIDD was up-regulated at 20 ℃, 17 ℃, and 14 ℃. DUSP3 was up-regulated at 20 ℃ but down-regulated at 17 ℃ and 14 ℃, and PARP was down-regulated at 17 ℃ and 14 ℃. JNK2 was up-regulated at 20 ℃ but down-regulated at 17 ℃ and 14 ℃. Our results suggest that DUSP could help inhibit apoptosis in the initial stage of cold stress, but low temperature could down-regulate it and up-regulate JNK-C-JUN, inducing apoptosis in a later stage. These data provide a basis for the study of the response mechanism of fish to cold.

Indexed as

MAP Kinase Signaling SystemMitogen-Activated Protein Kinase 8AnimalsApoptosisCold-Shock ResponseMitogen-Activated Protein Kinase 9PhosphorylationPoly(ADP-ribose) Polymerase InhibitorsMitogen-Activated Protein Kinase 8Mitogen-Activated Protein Kinase 9Poly(ADP-ribose) Polymerase InhibitorsApoptosisDUSPFishLow temperaturePampus argenteus

Identifiers

PMID37658990
OpenAlexW4386387808

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.