Evidence map›Paper›PMID 37658915›Full record

ArticleArchives of dermatological research2023

Genomic subtypes of cutaneous melanoma have distinct metabolic profiles: A single-cell transcriptomic analysis.

Michael J Diaz, Jasmine T Tran, Zi-Ning Choo, Kevin T Root, Sai Batchu, Silvija Milanovic, Lauren Ladehoff, Angela Fadil, Shari R Lipner

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In one paragraph

Article in Archives of dermatological research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 1 country.

Michael J DiazCollege of Medicine, University of Florida, Gainesville, FL, USA. michaeldiaz@ufl.edu.
Jasmine T TranSchool of Medicine, Indiana University, Indianapolis, IN, USA.
Zi-Ning ChooMedical College, Weill Cornell Medicine, New York, NY, USA.
Kevin T RootCollege of Medicine, University of Florida, Gainesville, FL, USA.
Sai BatchuCooper Medical School, Rowan University, Camden, NJ, USA.
Silvija MilanovicCollege of Medicine, University of Florida, Gainesville, FL, USA.
Lauren LadehoffMorsani College of Medicine, University of South Florida, Tampa, FL, USA.
Angela FadilCollege of Medicine, University of Florida, Gainesville, FL, USA.
Shari R LipnerDepartment of Dermatology, Weill Cornell Medicine, New York, NY, USA.
University of Florida · USCooper Medical School of Rowan University · USCornell University · USIndiana University – Purdue University Indianapolis · USUniversity of South Florida · USWeill Cornell Medicine · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveGenomic profiling previously classified melanoma into distinct subtypes based on the presence or absence of mutations in driver genes, but metabolic differences between and within these groups have yet to be thoroughly analyzed. Thus, the objective of the present study is to provide the first effort to holistically characterize the metabolic landscape of qualified melanoma genomic subtypes at single-cell resolution.

methodsExpression data for a total of 1145 malignant cells sourced from NRAS(Q61L), BRAF(V600E), and NRAS/BRAF WT melanomas were retrieved from the Broad Single Cell Portal. Metabolic activity was interrogated by pathway scoring and gene set enrichment analysis.

resultsA total of 53 metabolic pathways were differentially regulated in at least one melanoma genomic subtype. Some notable findings include: BRAF/NRAS WT cells were enriched for fatty acid biosynthesis and depleted for metabolism of alanine, aspartate, and glutamate; BRAF(V600E) melanoma cells were enriched for beta-alanine metabolism and depleted for phenylalanine metabolism; NRAS(Q61L) melanoma cells were enriched for steroid biosynthesis and depleted for linoleic acid metabolism.

conclusionPrimary limitations include the total quantity of single cells and breadth of available genomic subtypes plus inherent noisiness of the applied methodologies. Nonetheless, these findings nominate novel, testable therapeutic targets.

Indexed as

MelanomaSkin NeoplasmsCutaneous Malignant MelanomaGenomicsHumansMetabolomeMutationProto-Oncogene Proteins B-rafTranscriptomeProto-Oncogene Proteins B-rafGenomic subtypeMelanomaMetabolismOmicsPathway activitySingle-cell RNA sequencing

Identifiers

PMID37658915
OpenAlexW4386391453

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.