Evidence map›Paper›PMID 37658288›Full record

ArticleBMC genomics2023

Single-cell analysis of human prepuce reveals dynamic changes in gene regulation and cellular communications.

Fei Tan, Yuan Xuan, Lan Long, Yang Yu, Chunhua Zhang, Pengchen Liang, Yaoqun Wang, Meiyu Chen, Jiling Wen, Geng Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 72% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Fei Tan *School of Medicine, Shanghai Skin Disease Hospital, Tongji University, Shanghai, 200443, China. tanfeitrue@126.com.
Yuan Xuan *Shanghai Skin Disease Clinical College, The Fifth Clinical Medical College, Anhui Medical University, Shanghai Skin Disease Hospital, Shanghai, 200443, China.
Lan LongLonggang District Maternity & Child Healthcare Hospital of Shenzhen City, Shenzhen, 518172, China.
Yang YuDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Chunhua ZhangDepartment of Dermatology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, 201999, China.
Pengchen LiangSchool of Microelectronics, Shanghai University, Shanghai, 201800, China.
Yaoqun WangShanghai Skin Disease Clinical College, The Fifth Clinical Medical College, Anhui Medical University, Shanghai Skin Disease Hospital, Shanghai, 200443, China.
Meiyu ChenShanghai Skin Disease Clinical College, The Fifth Clinical Medical College, Anhui Medical University, Shanghai Skin Disease Hospital, Shanghai, 200443, China.
Jiling WenDepartment of Urology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China. wenjiling@tongji.edu.cn.
Geng ChenSchool of Medicine, Shanghai Skin Disease Hospital, Tongji University, Shanghai, 200443, China. chengeng66666@outlook.com.
Anhui Medical University · CNTongji University · CNShanghai Skin Disease Hospital · CNShanghai University · CNShenzhen Maternity and Child Healthcare Hospital · CN

Funding

Science and Technology Commission of Shanghai Municipality 22Y11905700Shanghai Municipal Health Commission 201940293
6 · The paper itself

Abstract

backgroundThe cellular and molecular dynamics of human prepuce are crucial for understanding its biological and physiological functions, as well as the prevention of related genital diseases. However, the cellular compositions and heterogeneity of human prepuce at single-cell resolution are still largely unknown. Here we systematically dissected the prepuce of children and adults based on the single-cell RNA-seq data of 90,770 qualified cells.

resultsWe identified 15 prepuce cell subtypes, including fibroblast, smooth muscle cells, T/natural killer cells, macrophages, vascular endothelial cells, and dendritic cells. The proportions of these cell types varied among different individuals as well as between children and adults. Moreover, we detected cell-type-specific gene regulatory networks (GRNs), which could contribute to the unique functions of related cell types. The GRNs were also highly dynamic between the prepuce cells of children and adults. Our cell-cell communication network analysis among different cell types revealed a set of child-specific (e.g., CD96, EPO, IFN-1, and WNT signaling pathways) and adult-specific (e.g., BMP10, NEGR, ncWNT, and NPR1 signaling pathways) signaling pathways. The variations of GRNs and cellular communications could be closely associated with prepuce development in children and prepuce maintenance in adults.

conclusionsCollectively, we systematically analyzed the cellular variations and molecular changes of the human prepuce at single-cell resolution. Our results gained insights into the heterogeneity of prepuce cells and shed light on the underlying molecular mechanisms of prepuce development and maintenance.

Indexed as

Endothelial CellsGene Expression RegulationAdultBone Morphogenetic ProteinsCell CommunicationGene Regulatory NetworksHumansSingle-Cell AnalysisBMP10 protein, humanBone Morphogenetic ProteinsCell–cell communicationsCellular dynamicsGene regulationHuman prepuceSingle-cell RNA-seq

Identifiers

PMID37658288
PMCPMC10474653
OpenAlexW4386385200

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.