Evidence map›Paper›PMID 37658191›Full record

ArticleOncogene2023

GREB1 isoform 4 is specifically transcribed by MITF and required for melanoma proliferation.

Koei Shinzawa, Shinji Matsumoto, Ryota Sada, Akikazu Harada, Kaori Saitoh, Keiko Kato, Satsuki Ikeda, Akiyoshi Hirayama, Kazunori Yokoi, Atsushi Tanemura and 4 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncogene, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Koei ShinzawaDepartment of Molecular Biology and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan. kshinzaw@gene.med.osaka-u.ac.jp.ORCID 0000-0003-3767-428X
Shinji MatsumotoDepartment of Molecular Biology and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.ORCID 0000-0003-1804-264X
Ryota SadaDepartment of Molecular Biology and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.ORCID 0000-0003-3403-0542
Akikazu HaradaDepartment of Molecular Biology and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.ORCID 0000-0002-8123-652X
Kaori SaitohInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.
Keiko KatoInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.
Satsuki IkedaInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.
Akiyoshi HirayamaInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.ORCID 0000-0002-0440-6820
Kazunori YokoiDepartment of Dermatology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.ORCID 0000-0003-0582-7325
Atsushi TanemuraDepartment of Dermatology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.ORCID 0000-0002-5239-8474
Keisuke NimuraDepartment of Genome Biology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.ORCID 0000-0001-9680-2646
Masahito IkawaResearch Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.ORCID 0000-0001-9859-6217
Tomoyoshi SogaInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.ORCID 0000-0001-9502-2509
Akira KikuchiDepartment of Molecular Biology and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan. akikuchi@cider.osaka-u.ac.jp.ORCID 0000-0003-3378-9522
The University of Osaka · JPKeio University · JPGunma University · JPOsaka Gakuin University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Growth regulation by estrogen in breast cancer 1 (GREB1) is involved in hormone-dependent and -independent tumor development (e.g., hepatoblastoma). In this study, we found that a GREB1 splicing variant, isoform 4 (Is4), which encodes C-terminal half of full-length GREB1, is specifically expressed via microphthalmia-associated transcription factor (MITF) in melanocytic melanoma, and that two MITF-binding E-box CANNTG motifs at the 5'-upstream region of GREB1 exon 19 are necessary for GREB1 Is4 transcription. MITF and GREB1 Is4 were strongly co-expressed in approximately 20% of the melanoma specimens evaluated (17/89 cases) and their expression was associated with tumor thickness. GREB1 Is4 silencing reduced melanoma cell proliferation in association with altered expression of cell proliferation-related genes in vitro. In addition, GREB1 Is4 targeting by antisense oligonucleotide (ASO) decreased melanoma xenograft tumor formation and GREB1 Is4 expression in a BRAF

Indexed as

MelanomaMicrophthalmia-Associated Transcription FactorAnimalsCell Line, TumorCell ProliferationGene Expression Regulation, NeoplasticHumansMiceNeoplasm ProteinsProtein IsoformsPyrimidinesGREB1 protein, humanMicrophthalmia-Associated Transcription FactorMITF protein, humanNeoplasm ProteinsProtein IsoformsPyrimidines

Identifiers

PMID37658191
PMCPMC10575781
OpenAlexW4386369826

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.