Evidence map›Paper›PMID 37656885›Full record

ReviewFEMS microbiology reviews2023

Methodologies for bacterial ribonuclease characterization using RNA-seq.

Laura Broglia, Anaïs Le Rhun, Emmanuelle Charpentier

Open access · hybridAbstract readReview
In one paragraph

Review in FEMS microbiology reviews, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 3 countries.

Laura BrogliaMax Planck Unit for the Science of Pathogens, D-10117 Berlin, Germany.ORCID 0000-0003-2099-7373
Anaïs Le RhunMax Planck Unit for the Science of Pathogens, D-10117 Berlin, Germany.
Emmanuelle CharpentierMax Planck Unit for the Science of Pathogens, D-10117 Berlin, Germany.ORCID 0000-0002-0254-0778
Centre National de la Recherche Scientifique · FRHumboldt-Universität zu Berlin · DEItalian Institute of Technology · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacteria adjust gene expression at the post-transcriptional level through an intricate network of small regulatory RNAs and RNA-binding proteins, including ribonucleases (RNases). RNases play an essential role in RNA metabolism, regulating RNA stability, decay, and activation. These enzymes exhibit species-specific effects on gene expression, bacterial physiology, and different strategies of target recognition. Recent advances in high-throughput RNA sequencing (RNA-seq) approaches have provided a better understanding of the roles and modes of action of bacterial RNases. Global studies aiming to identify direct targets of RNases have highlighted the diversity of RNase activity and RNA-based mechanisms of gene expression regulation. Here, we review recent RNA-seq approaches used to study bacterial RNases, with a focus on the methods for identifying direct RNase targets.

Indexed as

EndoribonucleasesRibonucleasesBacteriaRNARNA-SeqEndoribonucleasesRibonucleasesRNApost-transcriptional regulationRibonucleasesRNA decayRNA degradationRNA processingRNA sequencingtranscriptomics

Identifiers

PMID37656885
PMCPMC10503654
OpenAlexW4386348834

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.