Evidence map›Paper›PMID 37655276›Full record

ArticleCytotechnology2023

Assessing MTT and sulforhodamine B cell proliferation assays under multiple oxygen environments.

Ming Yao, Glenn Walker, Michael P Gamcsik

Open access · greenAbstract read
In one paragraph

Article in Cytotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Ming YaoDepartment of Mechanical and Aerospace Engineering, North Carolina State University, 1840 Entrepreneur Drive, Raleigh, NC 27695-7910 USA.
Glenn WalkerUNC/NCSU Joint Department of Biomedical Engineering, 1840 Entrepreneur Drive, Box 7115, Raleigh, NC 27695-7115 USA.
Michael P GamcsikUNC/NCSU Joint Department of Biomedical Engineering, 1840 Entrepreneur Drive, Box 7115, Raleigh, NC 27695-7115 USA.ORCID 0000-0002-0676-0110
North Carolina State University · USUNC/NCSU Joint Department of Biomedical Engineering · USUniversity of Mississippi · US

Funding

High-Throughput Screening Under Static or Dynamic HypoxiaR21CA202804 · NCI · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI GAMCSIK, MICHAEL · 2016 to 2018
$574k
NCI NIH HHS R21 CA202804
6 · The paper itself

Abstract

Cell proliferation can be measured directly by counting cells or indirectly using assays that quantitate total protein or metabolic activity. However, for comparing cell proliferation under varying oxygen conditions it is not clear that these assays are appropriate surrogates for cell counting as cell metabolism and protein synthesis may vary under different oxygen environments. We used permeable bottom tissue culture ware to compare proliferation assays as a function of static oxygen concentrations under oxygen partial pressure ( Supplementary Information: The online version contains supplementary material available at 10.1007/s10616-023-00584-0.

Indexed as

CancerHypoxiaMTT assayOxygen partial pressureProliferationSRB

Identifiers

PMID37655276
PMCPMC10465423
OpenAlexW4383217201

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.