ReviewChemical & biomedical imaging2023
Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy.
Review in Chemical & biomedical imaging, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The trial behind it
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Who cites it
11 citing papers in PubMed, 11 citations in OpenAlex.
- Molecular engineering of Cyanine-Hoechst hybrid materials for nanoscale DNA and chromatin imaging.JPhys materials · 2026Article
- Fused deep learning enables 6D single-molecule localization in polarization-resolved microscopy.Methods and applications in fluorescence · 2026Article
- Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes.Chemical & biomedical imaging · 2026Article
- SIMBA: an agentic AI platform for single-molecule multidimensional imaging.bioRxiv : the preprint server for biology · 2026Article
- Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy.bioRxiv : the preprint server for biology · 2026Article
- Functional super-resolution microscopy of fibers and polymers: convergence of artificial and biological systems at the nanoscale.Nanoscale horizons · 2026Review
- D‑Blur: A Deep Learning Approach for Mapping Subdiffraction Diffusion with Motion-Blurred Images.Chemical & biomedical imaging · 2025Article
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- Framework for Accurate Single-Molecule Spectroscopic Imaging Analyses Using Monte Carlo Simulation and Deep Learning.Analytical chemistry · 2025Article
- Photoswitchable Fluorescent Hydrazone for Super-Resolution Cell Membrane Imaging.Journal of the American Chemical Society · 2025Article
- Enhanced β-Amyloid Aggregation in Living Cells Imaged with Quinolinium-Based Spontaneous Blinking Fluorophores.Chemical & biomedical imaging · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Multidimensional single-molecule localization microscopy (mSMLM) represents a paradigm shift in the realm of super-resolution microscopy techniques. It affords the simultaneous detection of single-molecule spatial locations at the nanoscale and functional information by interrogating the emission properties of switchable fluorophores. The latter is finely tuned to report its local environment through carefully manipulated laser illumination and single-molecule detection strategies. This Perspective highlights recent strides in mSMLM with a focus on fluorophore designs and their integration into mSMLM imaging systems. Particular interests are the accomplishments in simultaneous multiplexed super-resolution imaging, nanoscale polarity and hydrophobicity mapping, and single-molecule orientational imaging. Challenges and prospects in mSMLM are also discussed, which include the development of more vibrant and functional fluorescent probes, the optimization of optical implementation to judiciously utilize the photon budget, and the advancement of imaging analysis and machine learning techniques.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.