Evidence map›Paper›PMID 37653390›Full record

ArticleJournal of orthopaedic surgery and research2023

ATF2-driven osteogenic activity of enoxaparin sodium-loaded polymethylmethacrylate bone cement in femoral defect regeneration.

Luobin Ding, Kangning Hao, Linchao Sang, Xiaoyu Shen, Ce Zhang, Dehao Fu, Xiangbei Qi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. ER-resident proteins are key players in cartilage and bone homeostasis.Frontiers in cell and developmental biology · 2025
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Luobin DingDepartment of Orthopedic Surgery, The Third Hospital of Hebei Medical University, No. 139, Ziqiang Road, Shijiazhuang, 050051, Hebei, People's Republic of China.
Kangning HaoDepartment of Orthopedic Surgery, The Third Hospital of Hebei Medical University, No. 139, Ziqiang Road, Shijiazhuang, 050051, Hebei, People's Republic of China.
Linchao SangDepartment of Orthopedic Surgery, Third Hospital of Shijiazhuang, Shijiazhuang, 050000, People's Republic of China.
Xiaoyu ShenDepartment of Orthopedic Surgery, The Third Hospital of Hebei Medical University, No. 139, Ziqiang Road, Shijiazhuang, 050051, Hebei, People's Republic of China.
Ce ZhangDepartment of Orthopedic Surgery, The Third Hospital of Hebei Medical University, No. 139, Ziqiang Road, Shijiazhuang, 050051, Hebei, People's Republic of China.
Dehao FuDepartment of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200000, People's Republic of China. fudehao@sjtu.edu.cn.
Xiangbei QiDepartment of Orthopedic Surgery, The Third Hospital of Hebei Medical University, No. 139, Ziqiang Road, Shijiazhuang, 050051, Hebei, People's Republic of China. qixiangbei1510@hebmu.edu.cn.
Third Hospital of Hebei Medical University · CNFirst Hospital of Shijiazhuang · CNHebei Medical University · CNShanghai Jiao Tong University · CN

Funding

Science and Technology Development Fund Projects of Central Government Guiding Local Government 226Z7720G
6 · The paper itself

Abstract

backgroundPolymethylmethacrylate (PMMA) bone cement loaded with enoxaparin sodium (PMMA@ES) has been increasingly highlighted to affect the bone repair of bone defects, but the molecular mechanisms remain unclear. We addressed this issue by identifying possible molecular mechanisms of PMMA@ES involved in femoral defect regeneration based on bioinformatics analysis and network pharmacology analysis.

methodsThe upregulated genes affecting the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) were selected through bioinformatics analysis, followed by intersection with the genes of ES-induced differentiation of BMSCs identified by network pharmacology analysis. PMMA@ES was constructed. Rat primary BMSCs were isolated and cultured in vitro in the proliferation medium (PM) and osteogenic medium (OM) to measure alkaline phosphatase (ALP) activity, mineralization of the extracellular matrix, and the expression of RUNX2 and OCN using gain- or loss-of-function experiments. A rat femoral bone defect model was constructed to detect the new bone formation in rats.

resultsATF2 may be a key gene in differentiating BMSCs into osteoblasts. In vitro cell assays showed that PMMA@ES promoted the osteogenic differentiation of BMSCs by increasing ALP activity, extracellular matrix mineralization, and RUNX2 and OCN expression in PM and OM. In addition, ATF2 activated the transcription of miR-335-5p to target ERK1/2 and downregulate the expression of ERK1/2. PMMA@ES induced femoral defect regeneration and the repair of femoral defects in rats by regulating the ATF2/miR-335-5p/ERK1/2 axis.

conclusionThe evidence provided by our study highlighted the ATF2-mediated mechanism of PMMA@ES in the facilitation of the osteogenic differentiation of BMSCs and femoral defect regeneration.

Indexed as

CalcinosisMicroRNAsAnimalsBone CementsCore Binding Factor Alpha 1 SubunitEnoxaparinOsteogenesisPolymethyl MethacrylateRatsBone CementsCore Binding Factor Alpha 1 SubunitEnoxaparinenoxaparin sodiumMicroRNAsPolymethyl MethacrylateATF2Bone marrow mesenchymal stem cellsEnoxaparin sodiumFemoral defect regenerationFemoral defect repairmiR-335-5pOsteogenic differentiationPolymethylmethacrylate bone cement

Identifiers

PMID37653390
PMCPMC10470168
OpenAlexW4386326638

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.