ArticleNature plants2023
Microbe-induced gene silencing boosts crop protection against soil-borne fungal pathogens.
Article in Nature plants, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Cross-kingdom RNA in plant-microbe interactions: from molecular interactions to next-generation crop protection.Stress biology · 2026Review
- Rational dsRNA design, scalable production and nanodelivery to enhance spray-induced gene silencing.Communications biology · 2026Review
- RNA regulation in plants.Science China. Life sciences · 2026Review
- Exploiting plant immune "switches" for resistance engineering.Stress biology · 2026Review
- Genome-wide identification of phosphate transporter gene family in Trichoderma harzianum and expression in response to biotic and abiotic stress.Scientific reports · 2026Article
- Plant viruses and the microbiome: a complex network shaping plant health and disease resistance.Archives of virology · 2026Review
- Cross-kingdom RNAi: a universal mechanism of inter-organismal communication with many unknowns.Journal of experimental botany · 2026Review
- Integrated Pest Management of Sclerotinia Stem Rot in Soybean: Current Strategies and Future Prospects.Journal of fungi (Basel, Switzerland) · 2025Review
- Article
- Plant-derived vesicle-like nanoparticles in food crops: emerging insights into nutritional biofortification and biomedical applications.Plant biotechnology journal · 2025Review
- Cross-kingdom RNAi induced by a beneficial endophytic fungus to its host requires transitivity and amplification of silencing signals.Plant biology (Stuttgart, Germany) · 2025Article
- Article
- Trans-Kingdom sRNA Silencing inPathogens (Basel, Switzerland) · 2025Review
- Circadian clock is critical for fungal pathogenesis by regulating zinc starvation response and secondary metabolism.Science advances · 2025Article
- Article
- Groundbreaking Technologies and the Biocontrol of Fungal Vascular Plant Pathogens.Journal of fungi (Basel, Switzerland) · 2025Review
- Epigenetic landscape underlying plant-microbiome chemical communication.The ISME journal · 2025Review
- Non-chemical control of fungal pathogens in crops: a one-health perspective on strategies, mechanisms, and future directions.Frontiers in plant science · 2025Review
- Leveraging plant-derived nanovesicles for advanced nucleic acid-based gene therapy.Theranostics · 2025Review
- Harnessing RNA interference for the control of Fusarium species: A critical review.Molecular plant pathology · 2024Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small RNA (sRNA)-mediated trans-kingdom RNA interference (RNAi) between host and pathogen has been demonstrated and utilized. However, interspecies RNAi in rhizospheric microorganisms remains elusive. In this study, we developed a microbe-induced gene silencing (MIGS) technology by using a rhizospheric beneficial fungus, Trichoderma harzianum, to exploit an RNAi engineering microbe and two soil-borne pathogenic fungi, Verticillium dahliae and Fusarium oxysporum, as RNAi recipients. We first detected the feasibility of MIGS in inducing GFP silencing in V. dahliae. Then by targeting a fungal essential gene, we further demonstrated the effectiveness of MIGS in inhibiting fungal growth and protecting dicotyledon cotton and monocotyledon rice plants against V. dahliae and F. oxysporum. We also showed steerable MIGS specificity based on a selected target sequence. Our data verify interspecies RNAi in rhizospheric fungi and the potential application of MIGS in crop protection. In addition, the in situ propagation of a rhizospheric beneficial microbe would be optimal in ensuring the stability and sustainability of sRNAs, avoiding the use of nanomaterials to carry chemically synthetic sRNAs. Our finding reveals that exploiting MIGS-based biofungicides would offer straightforward design and implementation, without the need of host genetic modification, in crop protection against phytopathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.