ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2023
Biosynthesis of High-Active Hemoproteins by the Efficient Heme-Supply Pichia Pastoris Chassis.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 49 citations in OpenAlex.
- Coupling Carbon-Source Transport with Antioxidant Biosynthesis in Yeast: Product Diversity and Engineering Strategies.Biology · 2026Review
- Production of Recombinant Hemoglobin inFoods (Basel, Switzerland) · 2026Article
- Metabolic engineering ofApplied and environmental microbiology · 2026Review
- The functional evaluation of Pichia pastoris hydrolysate as a protein source partial replacement of soybean meal in diets of growing-finishing pigs.Porcine health management · 2026Article
- Sustainable production of myoglobin meat protein in plant chloroplasts.Frontiers in plant science · 2026Article
- A Growth-Coupled Evolutionary Strategy Enhances Heme Biosynthesis in Saccharomyces cerevisiae.Biotechnology journal · 2026Article
- Subcellular Localization Dictates Therapeutic Function: Spatially Targeted Delivery of Amuc_1100 by EngineeredNutrients · 2025Article
- Cell-Free Production of Soybean Leghemoglobins and Nonsymbiotic Hemoglobin.ACS synthetic biology · 2025Article
- Mechanisms of Variation in Abdominal Adipose Color Among Male Kazakh Horses Through Non-Coding RNA Sequencing.Biology · 2025Article
- Metabolic engineering ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Compartmentalization of heme biosynthetic pathways into yeast mitochondria enhances heme production.NPJ science of food · 2025Article
- Article
- Metabolic engineering of yeast to efficiently synthesize heme and hemoproteins: recent advance and prospects.FEMS yeast research · 2025Review
- Biological Properties of Sandalwood Oil and Microbial Synthesis of Its Major Sesquiterpenoids.Biomolecules · 2024Review
- Biosynthesis of High-Active Hemoproteins by the Efficient Heme-Supply Pichia Pastoris Chassis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Microbial synthesis of valuable hemoproteins has become a popular research topic, and Pichia pastoris is a versatile platform for the industrial production of recombinant proteins. However, the inadequate supply of heme limits the synthesis of high-active hemoproteins. Here a strategy for enhancing intracellular heme biosynthesis to improve the titers and functional activities of hemoproteins is reported. After selecting a suitable expressional strategy for globins, the efficient heme-supply P. pastoris chassis is established by removing the spatial segregation during heme biosynthesis, optimizing precursor synthesis, assembling rate-limiting enzymes using protein scaffolds, and inhibiting heme degradation. This robust chassis produces several highly active hemoproteins, including porcine myoglobin, soy hemoglobin, Vitreoscilla hemoglobin, and P450-BM3, which can be used in the development of artificial meat, high-cell-density fermentation, and whole-cell catalytic synthesis of high-value-added compounds. Furthermore, the engineered chassis strain has great potential for producing and applying other hemoproteins with high activities in various fields.
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Registered trials
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