ArticleJournal of advanced research2024
The cotton MYB33 gene is a hub gene regulating the trade-off between plant growth and defense in Verticillium dahliae infection.
Article in Journal of advanced research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 27 citations in OpenAlex.
- The Regulatory Army of Plant Defense: Transcription Factors in the War for Plant Immunity.International journal of molecular sciences · 2026Review
- Ectopic Expression of ScALDH21 From a Desert Moss Enhances Cotton Resistance to Verticillium Wilt via the Modulation of Jasmonates and Phenylpropanoid Pathways.Plant biotechnology journal · 2026Article
- LjCIPK1: a CBL-interacting protein kinase fromFrontiers in plant science · 2026Article
- MYB transcription factors as context-dependent regulatory modules for precision crop improvement.Frontiers in plant science · 2026Article
- Transcriptome analysis reveals key pathways and regulatory networks involved in soybean's early resistance to Peronospora manshurica.BMC plant biology · 2025Article
- Ghr-miR166b-GhHB14 Pair With GhDLO1 Partner Mediates Cotton Plant Defence Against Verticillium dahliae.Molecular plant pathology · 2025Article
- Dual Transcriptome Analysis Reveals the Changes in Gene Expression in Both Cotton andJournal of fungi (Basel, Switzerland) · 2024Article
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Identification and characterization of circular RNAs involved in the fertility stability of cotton CMS-D2 restorer line under heat stress.BMC plant biology · 2024Article
- The ncRNAs Involved in the Regulation of Abiotic Stress-Induced Anthocyanin Biosynthesis in Plants.Antioxidants (Basel, Switzerland) · 2023Review
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionSessile plants engage in trade-offs between growth and defense capacity in response to fluctuating environmental cues. MYB is an important transcription factor that plays many important roles in controlling plant growth and defense. However, the mechanism behind how it keeps a balance between these two physiological processes is still largely unknown.
objectivesOur work focuses on the dissection of the molecular mechanism by which GhMYB33 regulates plant growth and defense.
methodsThe CRISPR/Cas9 technique was used to generate mutants for deciphering GhMYB33 functions. Yeast two-hybrid, luciferase complementary imaging, and co-immunoprecipitation assays were used to prove that proteins interact with each other. We used the electrophoretic mobility shift assay, yeast one-hybrid, and luciferase activity assays to analyze GhMYB33 acting as a promoter. A β-glucuronidase fusion reporter and 5' RNA ligase mediated amplification of cDNA ends analysis showed that ghr-miR319c directedly cleaved the GhMYB33 mRNA.
resultsOverexpressing miR319c-resistant GhMYB33 (rGhMYB33) promoted plant growth, accompanied by a significant decline in resistance against Verticillium dahliae. Conversely, its knockout mutant, ghmyb33, demonstrated growth restriction and concomitant augmentation of V. dahliae resistance. GhMYB33 was found to couple with the DELLA protein GhGAI1 and bind to the specific cis-elements of GhSPL9 and GhDFR1 promoters, thereby modulating internode elongation and plant resistance in V. dahliae infection. The ghr-miR319c was discovered to target and suppress GhMYB33 expression. The overexpression of ghr-miR319c led to enhanced plant resistance and a simultaneous reduction in plant height.
conclusionOur findings demonstrate that GhMYB33 encodes a hub protein and controls the expression of GhSPL9 and GhDFR1, implicating a pivotal role for the miR319c-MYB33 module to regulate the trade-offs between plant growth and defense.
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