Evidence map›Paper›PMID 37647213›Full record

ArticleACS applied bio materials2023

Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative.

Shirin Ilkhanizadeh, Aileen Gracias, Andreas K O Åslund, Marcus Bäck, Rozalyn Simon, Edel Kavanagh, Bianca Migliori, Christina Neofytou, Sven Nelander, Bengt Westermark and 8 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS applied bio materials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 3 institutions in 1 country.

Shirin IlkhanizadehDepartment of Neuroscience, Karolinska Institutet, Stockholm 171 77, Sweden.
Aileen GraciasDepartment of Neuroscience, Karolinska Institutet, Stockholm 171 77, Sweden.
Andreas K O ÅslundIFM, Department of Chemistry, Linköping University, Linköping 581 83, Sweden.ORCID 0000-0001-5222-9307
Marcus BäckIFM, Department of Chemistry, Linköping University, Linköping 581 83, Sweden.
Rozalyn SimonIFM, Department of Chemistry, Linköping University, Linköping 581 83, Sweden.
Edel KavanaghInstitute of Environmental Medicine, Karolinska Institutet, Stockholm 171 77, Sweden.
Bianca MiglioriDepartment of Neuroscience, Karolinska Institutet, Stockholm 171 77, Sweden.
Christina NeofytouDepartment of Neuroscience, Karolinska Institutet, Stockholm 171 77, Sweden.
Sven NelanderDepartment of Immunology, Genetics and Pathology, and Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, Uppsala 751 85, Sweden.
Bengt WestermarkDepartment of Immunology, Genetics and Pathology, and Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, Uppsala 751 85, Sweden.
Lene UhrbomDepartment of Immunology, Genetics and Pathology, and Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, Uppsala 751 85, Sweden.
Karin Forsberg-NilssonDepartment of Immunology, Genetics and Pathology, and Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, Uppsala 751 85, Sweden.
Peter KonradssonIFM, Department of Chemistry, Linköping University, Linköping 581 83, Sweden.
Ana I TeixeiraDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm 171 77, Sweden.ORCID 0000-0001-8169-8815
Per UhlénDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm 171 77, Sweden.
Bertrand JosephInstitute of Environmental Medicine, Karolinska Institutet, Stockholm 171 77, Sweden.
Ola HermansonDepartment of Neuroscience, Karolinska Institutet, Stockholm 171 77, Sweden.ORCID 0000-0001-9320-7921
K Peter R NilssonIFM, Department of Chemistry, Linköping University, Linköping 581 83, Sweden.
Karolinska Institutet · SELinköping University · SEUppsala University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There is an urgent need for simple and non-invasive identification of live neural stem/progenitor cells (NSPCs) in the developing and adult brain as well as in disease, such as in brain tumors, due to the potential clinical importance in prognosis, diagnosis, and treatment of diseases of the nervous system. Here, we report a luminescent conjugated oligothiophene (LCO), named p-HTMI, for non-invasive and non-amplified real-time detection of live human patient-derived glioblastoma (GBM) stem cell-like cells and NSPCs. While p-HTMI stained only a small fraction of other cell types investigated, the mere addition of p-HTMI to the cell culture resulted in efficient detection of NSPCs or GBM cells from rodents and humans within minutes. p-HTMI is functionalized with a methylated imidazole moiety resembling the side chain of histidine/histamine, and non-methylated analogues were not functional. Cell sorting experiments of human GBM cells demonstrated that p-HTMI labeled the same cell population as CD271, a proposed marker for stem cell-like cells and rapidly migrating cells in glioblastoma. Our results suggest that the LCO p-HTMI is a versatile tool for immediate and selective detection of neural and glioma stem and progenitor cells.

Indexed as

Brain NeoplasmsGlioblastomaNeural Stem CellsAdapaleneAdultBrainHumansAdapalenebioelectronicsbrain tumormethylationp75NTRprogenitor

Identifiers

PMID37647213
PMCPMC10521023
OpenAlexW4386287258

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.