Evidence map›Paper›PMID 37646268›Full record

ArticleJournal of diabetes2024

IFN-γ enhances the therapeutic efficacy of MSCs-derived exosome via miR-126-3p in diabetic wound healing by targeting SPRED1.

Wen Lu, Xuan Du, Shengyi Zou, Qionglei Fang, Mengjiao Wu, Huijuan Li, Bimin Shi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of diabetes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.

  1. Pooled it
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  9. Exosomes in Diabetic Wound Healing: Mechanisms, Applications, and Perspectives.Diabetes, metabolic syndrome and obesity : targets and therapy · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Wen LuDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Soochow University, Suzhou, China.
Xuan DuDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Soochow University, Suzhou, China.ORCID https://orcid.org/0000-0003-4584-7400
Shengyi ZouDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Soochow University, Suzhou, China.
Qionglei FangDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Soochow University, Suzhou, China.
Mengjiao WuDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Soochow University, Suzhou, China.
Huijuan LiDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Soochow University, Suzhou, China.
Bimin ShiDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Soochow University, Suzhou, China.ORCID https://orcid.org/0009-0006-7688-2283
Soochow University · CN

Funding

Key R&D Program of China 2019YFA0802400Suzhou Science and Technology SLT202008Youth Science and Technology Foundation of Suzhou City of China KJXW2022004
6 · The paper itself

Abstract

BACKGROUND AND

aimsThe traditional treatment of diabetic wounds is unsatisfactory. Exosomes isolated from bone marrow mesenchymal stem cells (BMSCs) promote the healing of diabetic wounds. However, whether the exosomes secreted by interferon (IFN)-γ-pretreated BMSCs have an enhanced therapeutic effect on diabetic wound healing and the relevant mechanisms remain unclear.

methodsIn this study, we isolated exosomes from the corresponding supernatants of BMSCs with (IExos) or without IFN-γ treatment (NExos). Human umbilical vein endothelial cells (HUVECs) were used to investigate the proliferation, migration, and tube formation under different treatments in vitro. Diabetic mice were induced by intraperitoneal administration of streptozotocin, and a circular full-thickness dermal defect was then made on the back of each mouse, followed by a multisite subcutaneous injection of phosphate buffered saline or exosomes. Hematoxylin-eosin (H&E) staining, Masson's trichrome staining, and histological analysis were performed to assess the speed and quality of wound healing.

resultsNExos treatment accelerated the healing of diabetic wounds by promoting angiogenesis in vivo and in vitro, and IExos exhibited superior therapeutic efficiency. MicroRNA (miR)-126-3p was significantly increased in IExos, and exosomal miR-126-3p promoted angiogenesis and diabetic wound healing via its transfer to HUVECs. miR-126-3p regulates SPRED1 by directly targeting the 3'-UTR. Mechanistically, IFN-γ-pretreated BMSCs secreted miR-126-3p-enriched exosomes, which enhanced the function of HUVECs and promoted angiogenesis via the SPRED1/Ras/Erk pathway.

conclusionExosomal miR-126-3p secreted from IFN-γ-pretreated BMSCs exhibited higher therapeutic efficacy than NExos in diabetic wound healing by promoting angiogenesis via the SPRED1/Ras/Erk axis.

Indexed as

Diabetes Mellitus, ExperimentalExosomesMicroRNAsAdaptor Proteins, Signal TransducingAnimalsCell ProliferationHumansHuman Umbilical Vein Endothelial CellsMiceWound HealingAdaptor Proteins, Signal TransducingMicroRNAsMIRN126 microRNA, humanSPRED1 protein, humandiabetic wound healingexosomesinterferon-γmesenchymal stem cellsmiR-126-3pSPRED1

Identifiers

PMID37646268
PMCPMC10809290
OpenAlexW4386271739

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.