Evidence map›Paper›PMID 37645720›Full record

ArticlebioRxiv : the preprint server for biology2023

Pneumatic Nano-Sieve for CRISPR-based Detection of Drug-resistant Bacteria.

Ruonan Peng, Xinye Chen, Fengjun Xu, Richard Hailstone, Yujie Men, Ke Du

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Ruonan PengDepartment of Chemical and Environmental Engineering, University of California, Riverside, 900 University Ave, Riverside, CA 92507, USA.
Xinye ChenDepartment of Chemical and Environmental Engineering, University of California, Riverside, 900 University Ave, Riverside, CA 92507, USA.
Fengjun XuDepartment of Chemical and Environmental Engineering, University of California, Riverside, 900 University Ave, Riverside, CA 92507, USA.
Richard HailstoneCenter for Imaging Science, Rochester Institute of Technology, 1 Lomb Memorial Dr, Rochester, NY 14623, USA.
Yujie MenDepartment of Chemical and Environmental Engineering, University of California, Riverside, 900 University Ave, Riverside, CA 92507, USA.
Ke DuDepartment of Chemical and Environmental Engineering, University of California, Riverside, 900 University Ave, Riverside, CA 92507, USA.
University of California, Riverside · USRochester Institute of Technology · US

Funding

Understand and Detect Sepsis: Pathogen Isolation, Biochemistry Assay, and Optofluidic SensingR35GM142763 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI DU, KE · 2021 to 2025
$2.0M
NIGMS NIH HHS R35 GM142763
6 · The paper itself

Abstract

The increasing prevalence of antibiotic-resistant bacterial infections, particularly methicillin-resistant Staphylococcus aureus (MRSA), presents a significant public health concern. Timely detection of MRSA is crucial to enable prompt medical intervention, limit its spread, and reduce antimicrobial resistance. Here, we introduce a miniaturized nano-sieve device featuring a pneumatically-regulated chamber for highly efficient MRSA purification from human plasma samples. By using packed magnetic beads as a filter and leveraging the deformability of the nano-sieve channel, we achieve an on-chip concentration factor of 15 for MRSA. We integrated this device with recombinase polymerase amplification (RPA) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas detection system, resulting in an on-chip limit of detection (LOD) of approximately 100 CFU/mL. This developed approach provides a rapid, precise, and centrifuge-free solution suitable for point-of-care diagnostics, with the potential to significantly improve patient outcomes in resource-limited medical conditions.

Indexed as

drug-resistant bacterialow-concentration detectionmicrofluidicsNano-sieveRPA/CRISPR-Cas

Identifiers

PMID37645720
PMCPMC10462146
OpenAlexW4386001050

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.