Evidence map›Paper›PMID 37641095›Full record

ArticleInfectious agents and cancer2023

Global comparative transcriptomes uncover novel and population-specific gene expression in esophageal squamous cell carcinoma.

Amal Alotaibi, Veerendra P Gadekar, Pranav Swaroop Gundla, Sumana Mandarthi, Nidhi Jayendra, Asna Tungekar, B V Lavanya, Ashok Kumar Bhagavath, Mary Anne Wong Cordero, Janne Pitkaniemi and 4 more

Open access · goldAbstract read
In one paragraph

Article in Infectious agents and cancer, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 4 countries.

Amal Alotaibi *Basic Science Department, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Veerendra P Gadekar *Mbiomics LLC, 16192 Coastal Highway, Lewes, DE, 19958, USA.
Pranav Swaroop GundlaMbiomics LLC, 16192 Coastal Highway, Lewes, DE, 19958, USA.
Sumana MandarthiMbiomics LLC, 16192 Coastal Highway, Lewes, DE, 19958, USA.
Nidhi JayendraMbiomics LLC, 16192 Coastal Highway, Lewes, DE, 19958, USA.
Asna TungekarMbiomics LLC, 16192 Coastal Highway, Lewes, DE, 19958, USA.
B V LavanyaMbiomics LLC, 16192 Coastal Highway, Lewes, DE, 19958, USA.
Ashok Kumar BhagavathDepartment of Cellular and Molecular Biology, University of Texas Health Science Center, Tyler, TX, USA.
Mary Anne Wong CorderoBasic Science Department, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Janne PitkaniemiFinnish Cancer Registry, Unioninkatu 22, 00130, Helsinki, Finland.
Shaik Kalimulla NiaziDepartment of Preparatory Health Sciences, Riyadh Elm University, Riyadh, Saudi Arabia.
Raghavendra UpadhyaManipal Center for Biotherapeutics Research, Manipal Academy of Higher Education, Manipal, India.
Asmatanzeem BepariBasic Science Department, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. ambepari@pnu.edu.sa.
Prashantha HebbarMbiomics LLC, 16192 Coastal Highway, Lewes, DE, 19958, USA. hebbar.prashantha@gmail.com.
Manipal Academy of Higher Education · INPrincess Nourah bint Abdulrahman University · SARiyadh Elm University · SAThe University of Texas Health Science Center at Tyler · USUniversity of Helsinki · FI

Funding

the Deanship of Scientific Research, Princess Nourah bint Abdulrahman University, through the Program of Research Project Funding After Publication 43- PRFA-P-8
6 · The paper itself

Abstract

backgroundEsophageal squamous cell carcinoma (ESCC) has a poor prognosis and is one of the deadliest gastrointestinal malignancies. Despite numerous transcriptomics studies to understand its molecular basis, the impact of population-specific differences on this disease remains unexplored.

aimsThis study aimed to investigate the population-specific differences in gene expression patterns among ESCC samples obtained from six distinct global populations, identify differentially expressed genes (DEGs) and their associated pathways, and identify potential biomarkers for ESCC diagnosis and prognosis. In addition, this study deciphers population specific microbial and chemical risk factors in ESCC.

methodsWe compared the gene expression patterns of ESCC samples from six different global populations by analyzing microarray datasets. To identify DEGs, we conducted stringent quality control and employed linear modeling. We cross-compared the resulting DEG lists of each populations along with ESCC ATLAS to identify known and novel DEGs. We performed a survival analysis using The Cancer Genome Atlas Program (TCGA) data to identify potential biomarkers for ESCC diagnosis and prognosis among the novel DEGs. Finally, we performed comparative functional enrichment and toxicogenomic analysis.

resultsHere we report 19 genes with distinct expression patterns among populations, indicating population-specific variations in ESCC. Additionally, we discovered 166 novel DEGs, such as ENDOU, SLCO1B3, KCNS3, IFI35, among others. The survival analysis identified three novel genes (CHRM3, CREG2, H2AC6) critical for ESCC survival. Notably, our findings showed that ECM-related gene ontology terms and pathways were significantly enriched among the DEGs in ESCC. We also found population-specific variations in immune response and microbial infection-related pathways which included genes enriched for HPV, Ameobiosis, Leishmaniosis, and Human Cytomegaloviruses. Our toxicogenomic analysis identified tobacco smoking as the primary risk factor and cisplatin as the main drug chemical interacting with the maximum number of DEGs across populations.

conclusionThis study provides new insights into population-specific differences in gene expression patterns and their associated pathways in ESCC. Our findings suggest that changes in extracellular matrix (ECM) organization may be crucial to the development and progression of this cancer, and that environmental and genetic factors play important roles in the disease. The novel DEGs identified may serve as potential biomarkers for diagnosis, prognosis and treatment.

Indexed as

CHRM3CREG2ENDOUESCCEsophagus cancerFHL1H2AC6Novel genes in ESCCToxicogenomics

Identifiers

PMID37641095
PMCPMC10463703
OpenAlexW4386225374

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.