ArticleBMC biotechnology2023
An effective device to enable consistent scratches for in vitro scratch assays.
Article in BMC biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Wound Healing Assay.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Multifunctional Thiolated Hyaluronic Acid-graft-PEDOT Dressing With Antifouling, On-Demand Drug Delivery and Electrical Stimulation for Wound Healing.Advanced healthcare materials · 2026Article
- The In Vitro Wound-Scratch Assay: Applications, Technical Advances, and Limitations in Wound Healing Research.International wound journal · 2026Review
- Synthesis and characterization of electroactive chitosan/gelatin/PEDOT:PSS hydrogels with mixed ionic-electronic conductivity for potential wound healing applications.RSC advances · 2026Article
- In vitro wound simulation: A high-throughput device for scratch assays.Methods (San Diego, Calif.) · 2026Article
- Scratching in style: 3D printers as plotters for automated and complex wound-healing assays.iScience · 2025Article
- In Vitro Wound Simulation: A High-Throughput Device for Scratch Assays.bioRxiv : the preprint server for biology · 2025Article
- Review
- Secretome from prolonged high-density human Wharton's jelly stem cell culture accelerates wound healing in both in vitro and in vivo models.International wound journal · 2025Article
- Dexketoprofen-Loaded Alginate-Grafted Poly(N-vinylcaprolactam)-Based Hydrogel for Wound Healing.International journal of molecular sciences · 2025Article
- A programmable platform for probing cell migration and proliferation.APL bioengineering · 2024Article
- Development and functional testing of a novel in vitro delayed scratch closure assay.Histochemistry and cell biology · 2024Article
- Fabrication of novel nanofiber composed of gelatin/alginate with zirconium oxide NPs regulate orthodontic progression of cartilage degeneration on Wnt/β-catenin signaling axis in MC3T3-E1 cells.Regenerative therapy · 2024Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe in-vitro scratch assay is a useful method in wound healing research to assess cell migration. In this assay, a scratch is created in a confluent cell layer by mechanically removing cells through manual scraping with a sharp-edged tool. This step is traditionally done with pipette tips and is unsuitable for high-throughput assays, as the created scratches are highly variable in width and position. Commercially available solutions are often expensive, and require specific cultureware which might not be suitable for all studies.
resultsIn this study, we have developed a flexible cell scratch device comprising a single wounding tool, a guide and an imaging template for consistent and reproducible scratch assays in 96-well plates. Our results showed that the device produced a more consistent scratch profile compared to the conventional method of using pipette tips. The imaging template also allowed operators to easily locate and image the same region of interest at different time points, which potentially could be used for other assays.
conclusionsOur flexible yet effective scratch device thus enables robust scratch assays that can be applied to different experimental needs, providing researchers with an easy and reliable tool for their studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.