ArticleThe ISME journal2023
Hydrogenotrophic methanogenesis is the key process in the obligately syntrophic consortium of the anaerobic ameba Pelomyxa schiedti.
Article in The ISME journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 23 citations in OpenAlex.
- Effects of ResB supplementation on gut microbiome composition in elderly pneumonia: a randomized pilot trial.European journal of clinical nutrition · 2026Article
- Integrative analysis of rumen microbiota activity and host metabolism following methanogenesis inhibition in dairy cattle.Microbiology spectrum · 2026Article
- Single-cell transcriptomics reveals lateral transfers of multiple functional genes from prokaryotes to free-living ciliated protists in detrital food webs.Marine life science & technology · 2026Article
- An extensive morphological and molecular characterization of the neglected class Odontostomatea (Ciliophora).Marine life science & technology · 2026Article
- Evolutionary assembly of a unique purple-green photosymbiosis revealed by expanded ciliate diversity.The ISME journal · 2026Article
- Biodegradable polylactic acid microplastics rewire the soil food web by impeding amoebae predation.The ISME journal · 2026Article
- Review
- New nucleolin-containing cytoplasmic bodies in an archamoebian protist Pelomyxa belevskii (Amoebozoa, Archamoebae, Pelobiontida).Protoplasma · 2025Article
- Navigating the Complex Terrain of Methane Synthesis: Multienzyme Control Points and Data-Driven Strategies.ACS omega · 2025Article
- Anaerobic breviate protist survival in microcosms depends on microbiome metabolic function.The ISME journal · 2025Article
- Lessons from Extremophiles: Functional Adaptations and Genomic Innovations across the Eukaryotic Tree of Life.Genome biology and evolution · 2024Article
- Rumen protozoa are a hub for diverse hydrogenotrophic functions.Environmental microbiology reports · 2024Article
- Methanogenic symbionts of anaerobic ciliates are host and habitat specific.The ISME journal · 2024Article
- Facultative endosymbiosis between cellulolytic protists and methanogenic archaea in the gut of the Formosan termiteISME communications · 2024Article
- Genomics of Preaxostyla Flagellates Illuminates the Path Towards the Loss of Mitochondria.PLoS genetics · 2023Article
- Diversity and taxonomic revision of methanogens and other archaea in the intestinal tract of terrestrial arthropods.Frontiers in microbiology · 2023Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pelomyxa is a genus of anaerobic amoebae that live in consortia with multiple prokaryotic endosymbionts. Although the symbionts represent a large fraction of the cellular biomass, their metabolic roles have not been investigated. Using single-cell genomics and transcriptomics, we have characterized the prokaryotic community associated with P. schiedti, which is composed of two bacteria, Candidatus Syntrophus pelomyxae (class Deltaproteobacteria) and Candidatus Vesiculincola pelomyxae (class Clostridia), and a methanogen, Candidatus Methanoregula pelomyxae. Fluorescence in situ hybridization and electron microscopy showed that Ca. Vesiculincola pelomyxae is localized inside vesicles, whereas the other endosymbionts occur freely in the cytosol, with Ca. Methanoregula pelomyxae enriched around the nucleus. Genome and transcriptome-based reconstructions of the metabolism suggests that the cellulolytic activity of P. schiedti produces simple sugars that fuel its own metabolism and the metabolism of a Ca. Vesiculincola pelomyxae, while Ca. Syntrophus pelomyxae energy metabolism relies on degradation of butyrate and isovalerate from the environment. Both species of bacteria and the ameba use hydrogenases to transfer the electrons from reduced equivalents to hydrogen, a process that requires a low hydrogen partial pressure. This is achieved by the third endosymbiont, Ca. Methanoregula pelomyxae, which consumes H
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