Evidence map›Paper›PMID 37632032›Full record

ArticleViruses2023

The Influence of the Omicron Variant on RNA Extraction and RT-qPCR Detection of SARS-CoV-2 in a Laboratory in Brazil.

Lívia Mara Silva, Lorena Rodrigues Riani, Juliana Brovini Leite, Jessica Mara de Assis Chagas, Laura Silva Fernandes, Romário Costa Fochat, Carmen Gomide Pinto Perches, Thiago César Nascimento, Lauren Hubert Jaeger, Marcelo Silva Silvério and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Comprehensive Review on Viral RNA Extraction Strategies for Enhanced Molecular Diagnostics.Interdisciplinary perspectives on infectious diseases · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Lívia Mara SilvaFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.
Lorena Rodrigues RianiFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.
Juliana Brovini LeiteFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.
Jessica Mara de Assis ChagasFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.
Laura Silva FernandesFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.
Romário Costa FochatFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.ORCID 0000-0003-0497-8164
Carmen Gomide Pinto PerchesHospital Universitário, Universidade Federal de Juiz de Fora, Av. Eugênio do Nascimento, s/n, Juiz de Fora 36038-330, MG, Brazil.
Thiago César NascimentoFaculdade de Enfermagem, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.ORCID 0000-0002-2304-7472
Lauren Hubert JaegerFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.ORCID 0000-0001-8031-7742
Marcelo Silva SilvérioFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.
Olavo Dos Santos Pereira-JúniorFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.
Frederico PittellaFaculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.ORCID 0000-0002-7847-9512
Universidade Federal de Juiz de Fora · BRHospital Universitário - Universidade Federal de Juiz de Fora · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The emergence of SARS-CoV-2 variants can affect their detection via RT-qPCR. The Omicron variant has a greater affinity for the upper respiratory system and causes clinical conditions similar to bronchitis, as opposed to the pneumonitis-like conditions caused by other SARS-CoV-2 variants. This characteristic increases the viscosity of clinical samples collected for diagnosis. Coinciding with the arrival of the Omicron variant, we observed a failure in control gene expression in our laboratory. In this report, we have optimized a rapid nucleic acid extraction step to restore gene expression and detect the presence of the SARS-CoV-2 virus. We reevaluated 3000 samples, compared variant types occurring in different time periods, and confirmed that the presence of the Omicron variant was responsible for changes observed in the characteristics of these clinical samples. For samples with large amounts of mucus, such as those containing the Omicron variant, a modification to the nucleic acid extraction step was sufficient to restore the quality of RT-qPCR results.

Indexed as

COVID-19Nucleic AcidsBrazilHumansRNASARS-CoV-2Nucleic AcidsRNAdiagnosticsOmicron variantRT-qPCRSARS-CoV-2

Identifiers

PMID37632032
PMCPMC10458054
OpenAlexW4385577823

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.