EWOD Chip with Micro-Barrier Electrode for Simultaneous Enhanced Mixing during Transportation.

Digital microfluidic platforms have been extensively studied in biology. However, achieving efficient mixing of macromolecules in microscale, low Reynolds number fluids remains a major challenge. To address this challenge, this study presents a novel design solution based on dielectric electro-wetting (EWOD) by optimizing the geometry of the transport electrode. The new design integrates micro-barriers on the electrodes to generate vortex currents that promote mixing during droplet transport. This design solution requires only two activation signals, minimizing the number of pins required. The mixing performance of the new design was evaluated by analyzing the degree of mixing inside the droplet and quantifying the motion of the internal particles. In addition, the rapid mixing capability of the new platform was demonstrated by successfully mixing the sorbitol solution with the detection solution and detecting the resulting reaction products. The experimental results show that the transfer electrode with a micro-barrier enables rapid mixing of liquids with a six-fold increase in mixing efficiency, making it ideal for the development of EWOD devices.