Evidence map›Paper›PMID 37630475›Full record

ArticleMicroorganisms2023

E7 Oncogene HPV58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis.

Bárbara Simas Chagas, Elias Tibúrcio Júnior, Ruany Cristyne de Oliveira Silva, Daffany Luana Dos Santos, Marconi Rego Barros Junior, Rita de Cássia Pereira de Lima, Maria da Conceição Viana Invenção, Vanessa Emanuelle Pereira Santos, Pedro Luiz França Neto, Antônio Humberto Silva Júnior and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Bárbara Simas ChagasLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.ORCID 0000-0003-3917-9055
Elias Tibúrcio JúniorLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
Ruany Cristyne de Oliveira SilvaLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
Daffany Luana Dos SantosLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
Marconi Rego Barros JuniorLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
Rita de Cássia Pereira de LimaLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
Maria da Conceição Viana InvençãoLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.ORCID 0000-0001-9360-1275
Vanessa Emanuelle Pereira SantosLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
Pedro Luiz França NetoLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
Antônio Humberto Silva JúniorCenter for Biological and Health Sciences, Federal University of Campina Grande, Campina Grande 58429-900, PB, Brazil.
Jacinto Costa Silva NetoLaboratory of Molecular and Cytological Research, Department of Histology, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
Marcus Vinícius de Aragão BatistaLaboratory of Molecular Genetics and Biotechnology, Department of Biology, Federal University of Sergipe, São Cristóvão 49100-000, SE, Brazil.ORCID 0000-0003-4745-8919
Antonio Carlos de FreitasLaboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.ORCID 0000-0002-4957-9549
Universidade Federal de Pernambuco · BRUniversidade Federal de Campina Grande · BRUniversidade Federal de Sergipe · BR

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior PNPD/CAPES 2020-2021FAPITEC/SE 00941/2018-5Fundação de Amparo à Ciência e Tecnologia de Pernambuco APQ-0562-2.02/14Fundação de Amparo à Ciência e Tecnologia de Pernambuco APQ-0748-2.02/17National Council for Scientific and Technological Development process 401202/2020-8National Council for Scientific and Technological Development process 401305/2014-7National Council for Scientific and Technological Development Process 447978/2014-4
6 · The paper itself

Abstract

Cervical cancer is associated with persistent infections by high-risk Human Papillomavirus (HPV) types that may have nucleotide polymorphisms and, consequently, different oncogenic potentials. Therefore, this study aimed to evaluate the genetic variability and structural effects of the E7 oncogene of HPV58 in cervical scraping samples from Brazilian women. The study was developed with patients from hospitals in the metropolitan area of Recife, PE, Brazil. The most frequent HPV types were, in descending order of abundance, HPV16, 31, and 58. Phylogenetic analysis demonstrated that the isolates were classified into sublineages A2, C1, and D2. Two positively selected mutations were found in E7: 63G and 64T. The mutations G41R, G63D, and T64A in the E7 protein reduced the stability of the protein structure. Utilizing an NF-kB reporter assay, we observed a decrease in the NK-kB pathway activity with the HPV58-E7 variant 54S compared to the WT E7. The other detected E7 HPV58 variants presented similar NF-kB pathway activity compared to the WT E7. In this study, it was possible to identify mutations that may interfere with the molecular interaction between the viral oncoproteins and host proteins.

Indexed as

E7 oncogenegene expressionhuman papillomavirus 58phylogenetic analysisvariants

Identifiers

PMID37630475
PMCPMC10458125
OpenAlexW4385346223

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.