Evidence map›Paper›PMID 37630168›Full record

ArticleMicromachines2023

Simultaneous Hydrostatic and Compressive Loading System for Mimicking the Mechanical Environment of Living Cartilage Tissue.

Minki Chang, Yosuke Takahashi, Kyosuke Miyahira, Yuma Omuro, Kevin Montagne, Ryusei Yamada, Junki Gondo, Yu Kambe, Masashi Yasuno, Noriyasu Masumoto and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micromachines, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Minki ChangDepartment of Bioengineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.ORCID 0000-0003-0568-0693
Yosuke TakahashiDepartment of Mechanical Engineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
Kyosuke MiyahiraDepartment of Mechanical Engineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
Yuma OmuroDepartment of Bioengineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
Kevin MontagneDepartment of Mechanical Engineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
Ryusei YamadaDepartment of Mechanical Engineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
Junki GondoDepartment of Mechanical Engineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
Yu KambeDepartment of Mechanical Engineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
Masashi YasunoDepartment of Mechanical Engineering, Faculty of Fundamental Engineering, Nippon Institute of Technology, Saitama 345-8501, Japan.
Noriyasu MasumotoDepartment of Mechanical Engineering, Faculty of Fundamental Engineering, Nippon Institute of Technology, Saitama 345-8501, Japan.
Takashi UshidaDepartment of Mechanical Engineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
Katsuko S FurukawaDepartment of Bioengineering, Graduate School of Engineering, University of Tokyo, Tokyo 113-8656, Japan.
The University of Tokyo · JPNippon Institute of Technology · JP

Funding

Japan Society for the Promotion of Science 19H01173Japan Society for the Promotion of Science 21H01383Japan Society for the Promotion of Science 21H05775Japan Society for the Promotion of Science 21K19889Japan Society for the Promotion of Science 23H03734
6 · The paper itself

Abstract

In vivo, articular cartilage tissue is surrounded by a cartilage membrane, and hydrostatic pressure (HP) and compressive strain increase simultaneously with the compressive stress. However, it has been impossible to investigate the effects of simultaneous loading in vitro. In this study, a bioreactor capable of applying compressive stress under HP was developed to reproduce ex vivo the same physical loading environment found in cartilage. First, a HP stimulation unit was constructed to apply a cyclic HP pressure-resistant chamber by controlling a pump and valve. A compression-loading mechanism that can apply compressive stress using an electromagnetic force was implemented in the chamber. The synchronization between the compression and HP units was evaluated, and the stimulation parameters were quantitatively evaluated. Physiological HP and compressive strain were applied to the chondrocytes encapsulated in alginate and gelatin gels after applying high HP at 25 MPa, which induced damage to the chondrocytes. It was found that compressive stimulation increased the expression of genes related to osteoarthritis. Furthermore, the simultaneous application of compressive strain and HP, which is similar to the physiological environment in cartilage, had an inhibitory effect on the expression of genes related to osteoarthritis. HP alone also suppressed the expression of osteoarthritis-related genes. Therefore, the simultaneous hydrostatic and compressive stress-loading device developed to simulate the mechanical environment in vivo may be an important tool for elucidating the mechanisms of disease onset and homeostasis in cartilage.

Indexed as

articular cartilagebioreactormechanical stimulation

Identifiers

PMID37630168
PMCPMC10456493
OpenAlexW4385979330

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.