Evidence map›Paper›PMID 37630057›Full record

ArticleMicromachines2023

Three-Dimensional Printing Enabled Droplet Microfluidic Device for Real-Time Monitoring of Single-Cell Viability and Blebbing Activity.

Meiai Lin, Ting Liu, Yeqian Liu, Zequan Lin, Jiale Chen, Jing Song, Yiya Qiu, Benqing Zhou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micromachines, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Meiai LinDepartment of Biomedical Engineering, College of Engineering, Shantou University, Shantou 515063, China.
Ting LiuDepartment of Biology, College of Science, Shantou University, Shantou 515063, China.
Yeqian LiuDepartment of Biomedical Engineering, College of Engineering, Shantou University, Shantou 515063, China.
Zequan LinDepartment of Biomedical Engineering, College of Engineering, Shantou University, Shantou 515063, China.
Jiale ChenDepartment of Biomedical Engineering, College of Engineering, Shantou University, Shantou 515063, China.
Jing SongDepartment of Biomedical Engineering, College of Engineering, Shantou University, Shantou 515063, China.
Yiya QiuDepartment of Biomedical Engineering, College of Engineering, Shantou University, Shantou 515063, China.
Benqing ZhouDepartment of Biomedical Engineering, College of Engineering, Shantou University, Shantou 515063, China.ORCID 0000-0003-1131-7591
Shantou University · CN

Funding

2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant 2020LKSFG07CGuangdong Basic and Applied Basic Foundation 2020A1515111053Guangdong Basic and Applied Basic Foundation 2022A1515011566
6 · The paper itself

Abstract

Droplet-based microfluidics with the characteristics of high throughput, low sample consumption, increasing reaction speed, and homogeneous volume control have been demonstrated as a useful platform for biomedical research and applications. The traditional fabrication methods of droplet microfluidics largely rely on expensive instruments, sophisticated operations, and even the requirement of an ultraclean room. In this manuscript, we present a 3D printing-based droplet microfluidic system with a specifically designed microstructure for droplet generation aimed at developing a more accessible and cost-effective method. The performance of droplet generation and the encapsulation capacity of the setup were examined. The device was further applied to measure the variation in cell viability over time and monitor the cell's blebbing activity to investigate its potential ability and feasibility for single-cell analysis. The result demonstrated that the produced droplets remained stable enough to enable the long-time detection of cell viability. Additionally, cell membrane protrusions featuring the life cycle of bleb initiation, expansion, and retraction can be well-observed. Three-dimensional printing-based droplet microfluidics benefit from the ease of manufacture, which is expected to simplify the fabrication of microfluidics and expand the application of the droplet approach in biomedical fields.

Indexed as

3D Printingcell blebbingcell viabilitydroplet microfluidics

Identifiers

PMID37630057
PMCPMC10456440
OpenAlexW4385422250

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.