ArticleAnimals : an open access journal from MDPI2023
Correlation between Targeted qPCR Assays and Untargeted DNA Shotgun Metagenomic Sequencing for Assessing the Fecal Microbiota in Dogs.
Article in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Using meta-analysis to understand the impacts of dietary protein and fat content on the composition of fecal microbiota of domestic dogs (Canis lupus familiaris): A pilot study.MicrobiologyOpen · 2024Pooled it
- Quantitative assessment of specific bacterial taxa of the intestinal microbiota of healthy and diarrheic horses.Journal of veterinary internal medicine · 2026Article
- Faecal Microbiota Transplantation Reduces Lesion Severity and Medication Use in Canine Atopic Dermatitis: A Randomised, Placebo-Controlled, Double-Blinded Clinical Trial.Veterinary dermatology · 2026Article
- Effect of low dose azithromycin on the fecal microbiome and fecal bile acids in healthy dogs.Journal of veterinary internal medicine · 2026Article
- Coprophagy in Healthy Dogs Does Not Alter Fecal Core Microbiota or Increase Carriage of Enteropathogens.Animals : an open access journal from MDPI · 2026Article
- Oral administration ofJournal of feline medicine and surgery · 2026Article
- Assessment of Fecal Microbiota in Healthy Dogs and Dogs with Cutaneous Mast Cell Tumors Treated with Electrochemotherapy Combined with Gene Electrotransfer of IL-12.Veterinary sciences · 2026Article
- The Role of Omics Technology in Evaluating Plastic Pollution's Effects on Plants: A Comprehensive Review.International journal of molecular sciences · 2025Review
- The fecal metabolome and microbiome are altered in dogs with idiopathic epilepsy compared to healthy dogs.Scientific reports · 2025Article
- Effects of meglumine antimoniate and allopurinol treatment on the fecal microbiome profile in dogs with leishmaniosis.Animal microbiome · 2025Article
- Effect of faecal microbial transplantation on clinical outcome, faecal microbiota and metabolome in dogs with chronic enteropathy refractory to diet.Scientific reports · 2025Article
- Effects of live Bacillus pumilus SG154 or a Lacticaseibacillus paracasei 327 postbiotic on apparent total tract nutrient digestibility and the immune function and fecal characteristics, metabolites, and microbiota of healthy adult dogs.Journal of animal science · 2025Article
- Effects of diet type on the core fecal bacterial taxa and the dysbiosis index of healthy adult dogs.Frontiers in veterinary science · 2025Article
- An insight into the gut microbiota of healthy and allergic West Highland Whiter Terrier dogs.PloS one · 2025Article
- Impact ofFrontiers in veterinary science · 2025Article
- Clinical Guidelines for Fecal Microbiota Transplantation in Companion Animals.Advances in small animal care · 2024Article
- Quantitative and dynamic profiling of human gut core microbiota by real-time PCR.Applied microbiology and biotechnology · 2024Article
- The Effect of Metronidazole versus a Synbiotic on Clinical Course and Core Intestinal Microbiota in Dogs with Acute Diarrhea.Veterinary sciences · 2024Article
- Temporal Variability of the Dominant Fecal Microbiota in Healthy Adult Cats.Veterinary sciences · 2024Article
- Species-level characterization of the core microbiome in healthy dogs using full-length 16S rRNA gene sequencing.Frontiers in veterinary science · 2024Article
Corrections and comments
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Authors and funding
22 authors at 10 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA shotgun sequencing is an untargeted approach for identifying changes in relative abundances, while qPCR allows reproducible quantification of specific bacteria. The canine dysbiosis index (DI) assesses the canine fecal microbiota by using a mathematical algorithm based on qPCR results. We evaluated the correlation between qPCR and shotgun sequencing using fecal samples from 296 dogs with different clinical phenotypes. While significant correlations were found between qPCR and sequencing, certain taxa were only detectable by qPCR and not by sequencing. Based on sequencing, less than 2% of bacterial species (17/1190) were consistently present in all healthy dogs (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.