ArticleGenome biology2023
Single-cell resolution analysis reveals the preparation for reprogramming the fate of stem cell niche in cotton lateral meristem.
Article in Genome biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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30 citing papers in PubMed, 57 citations in OpenAlex.
- A spatiotemporal single-cell transcriptomic atlas reveals fertilization-induced auxin signaling driving strawberry fruit set and development.Nature communications · 2026Article
- GhNAC47 activates GhSKS6-mediated cell wall remodeling to promote cotton chemical defoliation.The New phytologist · 2026Article
- Single-cell insights into plant growth, adaptation, and evolution.Journal of integrative plant biology · 2026Review
- Genome-Wide Identification and Expression Analysis of theInternational journal of molecular sciences · 2026Article
- Endogenous Multilayer Control of Cambial Stem Cells and Its Consequences for Wood Formation.Plants (Basel, Switzerland) · 2026Review
- Integrated experimental and computational workflows for single-cell transcriptomics in plants.Plant methods · 2026Article
- Comparative single-cell transcriptomic map reveals divergence in leaves between two cotton species at cell type resolution.Journal of advanced research · 2026Article
- MONOPTEROS isoform MP11ir plays a role during somatic embryogenesis in Arabidopsis thaliana.Plant physiology · 2025Article
- Woody Plant Transformation: Current Status, Challenges, and Future Perspectives.Plants (Basel, Switzerland) · 2025Review
- Decoding cell fate: integrated experimental and computational analysis at the single-cell level.Bioinformatics (Oxford, England) · 2025Review
- Walnut genome editing: an optimized CRISPR/Cas9 platform with superior genotypes and endogenous promoters.Horticulture research · 2025Article
- Genome assembly of two allotetraploid cotton germplasms reveals mechanisms of somatic embryogenesis and enables precise genome editing.Nature genetics · 2025Article
- Highly Efficient Regeneration ofPlants (Basel, Switzerland) · 2025Article
- Breeding triple-advantage cottonseed with higher yield, enhanced nutrition, and reduced toxicity by redirecting terpenoid metabolism to astaxanthin.Plant biotechnology journal · 2025Article
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- Global landscape of protein phosphorylation during plant regeneration initiation in cotton (Gossypium hirsutum L.).BMC biology · 2025Article
- Opportunities and challenges in the application of single-cell transcriptomics in plant tissue research.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Construction of a Cas9-targeted mutagenesis mini-library in the upland cotton genetic standard line (TM-1).Plant cell reports · 2025Article
- Integrated RNA-seq and snRNA-seq analysis identifiesFrontiers in plant science · 2025Article
- Unlocking regeneration potential: harnessing morphogenic regulators and small peptides for enhanced plant engineering.The Plant journal : for cell and molecular biology · 2025Review
Corrections and comments
- Erratum issued
Authors and funding
15 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSomatic embryogenesis is a major process for plant regeneration. However, cell communication and the gene regulatory network responsible for cell reprogramming during somatic embryogenesis are still largely unclear. Recent advances in single-cell technologies enable us to explore the mechanism of plant regeneration at single-cell resolution.
resultsWe generate a high-resolution single-cell transcriptomic landscape of hypocotyl tissue from the highly regenerable cotton genotype Jin668 and the recalcitrant TM-1. We identify nine putative cell clusters and 23 cluster-specific marker genes for both cultivars. We find that the primary vascular cell is the major cell type that undergoes cell fate transition in response to external stimulation. Further developmental trajectory and gene regulatory network analysis of these cell clusters reveals that a total of 41 hormone response-related genes, including LAX2, LAX1, and LOX3, exhibit different expression patterns in the primary xylem and cambium region of Jin668 and TM-1. We also identify novel genes, including CSEF, PIS1, AFB2, ATHB2, PLC2, and PLT3, that are involved in regeneration. We demonstrate that LAX2, LAX1 and LOX3 play important roles in callus proliferation and plant regeneration by CRISPR/Cas9 editing and overexpression assay.
conclusionsThis study provides novel insights on the role of the regulatory network in cell fate transition and reprogramming during plant regeneration driven by somatic embryogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.