ArticleNature communications2023
Infrared spectroscopic laser scanning confocal microscopy for whole-slide chemical imaging.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Computational Mass Spectrometry Imaging in the Era of AI.Chemical reviews · 2026Review
- Fix or freeze? Spectral differences arising from tissue preparation in chemical imaging.The Analyst · 2026Article
- Cohort-Scale Spatial Autocorrelation for Tumor Prediction in Mid-Infrared Pathology and Spatial Biomarker Discovery Using MALDI Imaging Lipidomics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Denoising and Baseline Correction of Low-Scan FTIR Spectra: A Benchmark of Deep Learning Models Against Traditional Signal Processing.Bioengineering (Basel, Switzerland) · 2026Article
- Mid-Infrared Transparent Materials: from Mechanisms to Cutting-Edge Applications.Nano-micro letters · 2026Review
- Fix or Freeze? Spectral Differences Arising from Tissue Preparation in Chemical Imaging.bioRxiv : the preprint server for biology · 2025Article
- Mid-Infrared Photothermal Mesoscopy with Millimeter Field of View and Sub-micron Spatial Resolution.bioRxiv : the preprint server for biology · 2025Article
- Combining quantum cascade lasers and plasmonic metasurfaces to monitorNanophotonics (Berlin, Germany) · 2025Article
- Monitoring Molecular Uptake and Cancer Cells' Response by Development of Quantitative Drug Derivative Probes for Chemical Imaging.Analytical chemistry · 2025Article
- Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy.Nature communications · 2025Article
- Enabling Chemical Holography: A Comprehensive Framework and Implementation.IEEE photonics journal · 2025Article
- Deep MALDI-MS spatial omics guided by quantum cascade laser mid-infrared imaging microscopy.Nature communications · 2025Article
- Wide-field bond-selective fluorescence imaging: from single-molecule to cellular imaging beyond video rate.Optica · 2025Article
- Rapid Hyperspectral Photothermal Mid-Infrared Spectroscopic Imaging from Sparse Data for Gynecologic Cancer Tissue Subtyping.Analytical chemistry · 2024Article
- Cervical Cancer Tissue Analysis Using Photothermal Midinfrared Spectroscopic Imaging.Chemical & biomedical imaging · 2024Article
- Machine learning-assisted mid-infrared spectrochemical fibrillar collagen imaging in clinical tissues.Journal of biomedical optics · 2024Article
- Inspiring a convergent engineering approach to measure and model the tissue microenvironment.Heliyon · 2024Review
- Overtone photothermal microscopy for high-resolution and high-sensitivity vibrational imaging.Nature communications · 2024Article
- Vibrational imaging of metabolites for improved microbial cell strains.Journal of biomedical optics · 2024Review
- Machine learning assisted mid-infrared spectrochemical fibrillar collagen imaging in clinical tissues.bioRxiv : the preprint server for biology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 3 institutions in 1 country.
Funding
Abstract
Chemical imaging, especially mid-infrared spectroscopic microscopy, enables label-free biomedical analyses while achieving expansive molecular sensitivity. However, its slow speed and poor image quality impede widespread adoption. We present a microscope that provides high-throughput recording, low noise, and high spatial resolution where the bottom-up design of its optical train facilitates dual-axis galvo laser scanning of a diffraction-limited focal point over large areas using custom, compound, infinity-corrected refractive objectives. We demonstrate whole-slide, speckle-free imaging in ~3 min per discrete wavelength at 10× magnification (2 μm/pixel) and high-resolution capability with its 20× counterpart (1 μm/pixel), both offering spatial quality at theoretical limits while maintaining high signal-to-noise ratios (>100:1). The data quality enables applications of modern machine learning and capabilities not previously feasible - 3D reconstructions using serial sections, comprehensive assessments of whole model organisms, and histological assessments of disease in time comparable to clinical workflows. Distinct from conventional approaches that focus on morphological investigations or immunostaining techniques, this development makes label-free imaging of minimally processed tissue practical.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.