ArticleCell stem cell2023
Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells.
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Article in Cell stem cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37citing papers in PubMed
15.3field-weighted citation impact, top 1% of its field
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37 citing papers in PubMed, 59 citations in OpenAlex.
- Preclinical human models of primary ciliary dyskinesia.European respiratory review : an official journal of the European Respiratory Society · 2026Review
- Standardized pipeline for establishing, expanding, and differentiating airway and alveolar organoids from human BAL fluid.American journal of physiology. Lung cellular and molecular physiology · 2026Article
- Regenerative therapeutics for chronic obstructive pulmonary disease.Pharmacological reviews · 2026Review
- Spatial Distribution of K13-Positive Airway Epithelial Cells in Idiopathic Pulmonary Fibrosis.Biomedicines · 2026Article
- Tracheal Regeneration: Recent Progress in the Application of Stem Cells in Tracheal Bioengineering.International journal of molecular sciences · 2026Review
- A serum-free culture medium for long-term expansion of human airway basal stem cells.Stem cell research & therapy · 2026Article
- Stem cell-based therapies for silicosis: mechanisms, sources, clinical translation, and emerging strategies.Frontiers in immunology · 2026Review
- Crosstalk between airway epithelial cells and mast cells in airway inflammation.Respiratory research · 2025Review
- Standardized pipeline for establishing, expanding, and differentiating airway and alveolar organoids from human BAL fluid.bioRxiv : the preprint server for biology · 2025Article
- Airway Basal Stem Cell Population Is Enlarged in Bronchial Thermoplasty Treated Airways in Severe Asthma Patients.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2025Article
- A stem cell-based platform for functional analysis of genetic variants in lung disease.bioRxiv : the preprint server for biology · 2025Article
- Multiplexed self-adaptable Janus hydrogels rescue epithelial malfunction to promote complete trachea repair.Nature communications · 2025Article
- A Differentiated Epithelial Layer Graft Improves Fibrosis and Survival in Airway Stenosis via IL-37.Allergy · 2025Article
- Ferrostatin-1 inhibits tracheal basal cell ferroptosis to facilitate the rapid epithelization of 3D-printed tissue-engineered tracheas.Stem cell research & therapy · 2025Article
- Engineered hydrogel biomaterials facilitate lung progenitor cell differentiation from induced pluripotent stem cells.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- Life-long functional regeneration of in vivo airway epithelium by the engraftment of airway basal stem cells.Nature protocols · 2025Review
- Specialized Pulmonary Vascular Cells in Development and Disease.Annual review of physiology · 2025Review
- Airway basal stem cell therapy for lung diseases: an emerging regenerative medicine strategy.Stem cell research & therapy · 2025Review
- Airway Disease Modeling with Gene-Edited Human Basal Cell Transplantation.American journal of respiratory and critical care medicine · 2025Article
- Recent Advancements in the Generation and Application of Therapeutic Cell Populations for Lung Epithelial Repair.Journal of tissue engineering and regenerative medicine · 2025Review
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Authors and funding
17 authors at 3 institutions in 1 country.
Liang MaCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Bibek R ThapaCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA; Department of Biology, Boston University, Boston, MA 02215, USA.
Jake A Le SuerCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Andrew Tilston-LünelThe Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA; Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA.
Michael J HerrigesCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Andrew BericalCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Mary Lou BeermannCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Feiya WangCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
Pushpinder S BawaCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
Anat KohnCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Alexandra B YsasiCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Hirofumi KiyokawaCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Taylor M MatteCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Scott H RandellMarsico Lung Institute/Cystic Fibrosis Center, Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Xaralabos VarelasDepartment of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA.
Finn J HawkinsCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Darrell N KottonCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA. Electronic address: dkotton@bu.edu.
Boston Medical Center · USBoston University · USUniversity of North Carolina at Chapel Hill · US
Funding
Vector CoreP30DK065988 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Martina Gentzsch · 2004 to 2026
$26.5MBIOLOGY OF THE LUNG--MULTIDISCIPLINARY PROGRAMT32HL007035 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Darrell N. Kotton, JOSEPH P MIZGERD · 1985 to 2026
$24.3MEditing Alveolar Progenitor Cells for Correction of Monogenic DiseaseU01HL134745 · NHLBI · CINCINNATI CHILDRENS HOSP MED CTR · PI KOTTON, DARRELL N., MORRISEY, EDWARD E · 2016 to 2022
$8.3MEpithelial stem/progenitor cells as repair agents in diffuse alveolar damageU01HL134766 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHAPMAN, HAROLD A, KOTTON, DARRELL N. · 2016 to 2022
$8.1MPCTC Administrative Coordinating CenterU24HL134763 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI ALBRECHT, JENNIFER S., TERRIN, MICHAEL L · 2016 to 2022
$7.9MDerivation of Transplantable Lung Epithelial Progenitors from iPS CellsR01HL095993 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Darrell N. Kotton · 2009 to 2026
$7.5MDefining epithelial polarity cues that direct cell fateR01HL124392 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI VARELAS, XARALABOS · 2014 to 2024
$4.5MNATIONAL BIOREPOSITORY OF LUNG DISEASE-SPECIFIC HUMAN INDUCED PLURIPOTENT STEM CELLS:THE PURPOSE OF THIS RESOURCE IS TO BUILD AND SUPPORT A CRITICAL75N92020C00005 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI N/A, N/A · 2021 to 2024
$2.6MiPSC-Derived Airway Basal Cells to Model Human Airway Development and DiseaseR01HL139799 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI HAWKINS, FINN · 2018 to 2022
$2.1MGeneration of functional lung stem cells from human iPSCsU01HL148692 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Finn Hawkins, Darrell N. Kotton · 2019 to 2026
$2.0MHigh Sensitivity In Vivo Imaging System with Integrated Micro-Computed Tomography for Animal Phenotyping CoreS10OD030501 · OD · BOSTON UNIVERSITY MEDICAL CAMPUS · PI SETA, FRANCESCA · 2022 to 2022
$600kDefining non-nuclear roles for Yap in the development of multi-ciliated cellsR21HD094012 · NICHD · BOSTON UNIVERSITY MEDICAL CAMPUS · PI VARELAS, XARALABOS · 2018 to 2019
$454kNHLBI NIH HHS 75N92020C00005NHLBI NIH HHS R01 HL095993NHLBI NIH HHS R01 HL124392NHLBI NIH HHS R01 HL139799NHLBI NIH HHS T32 HL007035NHLBI NIH HHS U01 HL134745NHLBI NIH HHS U01 HL134766NHLBI NIH HHS U01 HL148692NHLBI NIH HHS U24 HL134763NICHD NIH HHS R21 HD094012NIDDK NIH HHS P30 DK065988NIH HHS S10 OD030501
Abstract
Life-long reconstitution of a tissue's resident stem cell compartment with engrafted cells has the potential to durably replenish organ function. Here, we demonstrate the engraftment of the airway epithelial stem cell compartment via intra-airway transplantation of mouse or human primary and pluripotent stem cell (PSC)-derived airway basal cells (BCs). Murine primary or PSC-derived BCs transplanted into polidocanol-injured syngeneic recipients give rise for at least two years to progeny that stably display the morphologic, molecular, and functional phenotypes of airway epithelia. The engrafted basal-like cells retain extensive self-renewal potential, evident by the capacity to reconstitute the tracheal epithelium through seven generations of secondary transplantation. Using the same approach, human primary or PSC-derived BCs transplanted into NOD scid gamma (NSG) recipient mice similarly display multilineage airway epithelial differentiation in vivo. Our results may provide a step toward potential future syngeneic cell-based therapy for patients with diseases resulting from airway epithelial cell damage or dysfunction.
Indexed as
Pluripotent Stem CellsAnimalsCell- and Tissue-Based TherapyEpithelial CellsEpitheliumHumansMiceMice, Inbred NODMice, SCIDairway basal cellsairway epithelial stem cell transplantationdirected differentiationpluripotent stem cellsregeneration
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